Intra- and interspecific molecular polymorphism of thrips species

被引：15

作者：

Bayar, K

Törjék, O

Kiss, E

Gyulai, G

Heszky, L

机构：

[1] Szent Istvan Univ, Dept Plant Protect, H-2103 Godollo, Hungary

[2] Szent Istvan Univ, Dept Genet & Plant Breeding, H-2103 Godollo, Hungary

来源：

ACTA BIOLOGICA HUNGARICA | 2002年 / 53卷 / 03期

关键词：

molecular polymorphism; RAPD; Aeolothripidae; Thripidae; Thysanoptera;

D O I：

10.1556/ABiol.53.2002.3.8

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Molecular polymorphism of six species of Thysanoptera of both sexes, collected from different locations and host plants in Hungary was studied by using RAPD-PCR technique. The specimens were classified according to sampling sites (Godollo, Nagykovacsi mid Valko), host plants (Lathyrus tuberosus, Medieago sativa, Taraxacum officinale, Trifolium pratense), sexes, and larvae in case of Aeolothrips intermedius. On the basis of the total of 103 fragments generated by 15 RAPD primers the genetic distances were calculated by cluster analysis using simple matching method. The dendrogram resulted in two main groups: Aeolothripidae (Aeolothrips intermedius) and Thripidae (Frankliniella intonsa, Kakothrips robustus, Odonlothrips confusus, Thrips dilatatus and T. tabaci). Within the family Thripidae two subgroups were observed including (i) F. intonsa, T. dilatatus and T. tabaci, and (ii) K. robustus and O. confusus. Two population-specific and one sex-linked fragments were identified by the RAPD primers, OPQ14, NO11 and OPA08, respectively.

引用

页码：317 / 324

页数：8

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