New Horizons of Genomic in situ Hybridization

被引：34

作者：

Markova, M. ^{[1
]}

Vyskot, B. ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Biophys, Dept Plant Dev Genet, CZ-61265 Brno, Czech Republic

来源：

CYTOGENETIC AND GENOME RESEARCH | 2009年 / 126卷 / 04期

关键词：

Evolution; Genomic in situ hybridization; Phylogenetics; MOLECULAR CYTOGENETIC CHARACTERIZATION; CHROMOSOME IDENTIFICATION; REPETITIVE SEQUENCES; SILENE-LATIFOLIA; INTERSPECIFIC HYBRID; ARABIDOPSIS-THALIANA; HEXAPLOID WHEAT; SEX-CHROMOSOMES; BRASSICA-NAPUS; C-GENOME;

D O I：

10.1159/000275796

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This review summarizes conventional and recent applications of genomic in situ hybridization (GISH). GISH is a well recognized technique, but its modifications and applications have not been widely used. Here, we show how modifications to the GISH technique can be used as tools to 'paint' plant chromosomes. In addition, we describe novel applications, e. g. how GISH banding could be used for karyotyping plant chromosomes. We further discuss recent phylogenetic applications of GISH that allow a semiquantitative signal analysis and the possibility of comparing and combining this cytogenetic technique with DNA sequence-based phylogenetic trees. Copyright (C) 2010 S. Karger AG, Basel

引用

页码：368 / 375

页数：8

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