Redefining the concept of reactive astrocytes as cells that remain within their unique domains upon reaction to injury

被引:466
作者
Wilhelmsson, Ulrika
Bushongt, Eric A.
Price, Diana L.
Smarr, Benjamin L.
Phung, Van
Terada, Masako
Ellisman, Mark H.
Pekny, Milos
机构
[1] Univ Gothenburg, Dept Clin Neurosci & Rehabil, Inst Neurosci & Physiol, Sahlgrenska Acad, SE-40530 Gothenburg, Sweden
[2] Univ Calif San Diego, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA
关键词
astrocyte domains; astrocyte hypertrophy;
D O I
10.1073/pnas.0602841103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Reactive astrocytes in neurotrauma, stroke, or neurodegeneration are thought to undergo cellular hypertrophy, based on their morphological appearance revealed by immunohistochemical detection of glial fibrillary acidic protein, vimentin, or nestin, all of them forming intermediate filaments, a part of the cytoskeleton. Here, we used a recently established dye-filling method to reveal the full three-dimensional shape of astrocytes assessing the morphology of reactive astrocytes in two neurotrauma models. Both in the denervated hippocampal region and the lesioned cerebral cortex, reactive astrocytes increased the thickness of their main cellular processes but did not extend to occupy a greater volume of tissue than nonreactive astrocytes. Despite this hypertrophy of glial fibrillary acidic protein-containing cellular processes, interdigitation between adjacent hippocampal astrocytes remained minimal. This work helps to redefine the century-old concept of hypertrophy of reactive astrocytes.
引用
收藏
页码:17513 / 17518
页数:6
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