Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein

被引：15

作者：

Blance, SJ ^{[1
]}

Williams, NL ^{[1
]}

Preston, ZA ^{[1
]}

Bishara, J ^{[1
]}

Smyth, MS ^{[1
]}

Maxwell, A ^{[1
]}

机构：

[1] Univ Leicester, Dept Biochem, Leicester LE1 7RH, Leics, England

来源：

PROTEIN SCIENCE | 2000年 / 9卷 / 05期

关键词：

coumarin; gyrase; novobiocin; suppressor; topoisomerase;

D O I：

10.1110/ps.9.5.1035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for suppressors of the ts phenotype yielded second-site mutations in GyrB at His38 and Thr157. The properties of proteins bearing these mutations have been characterized, and a mechanism of suppression is proposed based upon structural considerations.

引用

页码：1035 / 1037

页数：3

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