G protein activation elicits cerebrovasodilation through interaction with KATP and KCa channels

被引:7
作者
Armstead, WM
机构
[1] Univ Penn, Dept Anesthesia, Philadelphia, PA 19104 USA
[2] Univ Penn, Dept Pharmacol, Philadelphia, PA 19104 USA
基金
美国国家卫生研究院;
关键词
newborn; cerebral circulation; K+ channel; signal transduction;
D O I
10.1016/S0006-8993(02)03698-3
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
This study was designed to characterize the vascular activity of two activators of a pertussis toxin-sensitive G protein, mastoparan and mastoparan-7, on pial artery diameter and their interaction with the ATP sensitive K (K-ATP) and calcium sensitive K (K-Ca) channel in newborn pigs equipped with a closed cranial window. Mastoparan (10(-8), 10(-6) M) elicited pial artery dilation that was blunted by the K-ATP channel antagonist glibenclamide (10-6 M) and attenuated by the K-Ca channel antagonist iberiotoxin (10(-7) M) (10+/-1 and 17+/-1 vs. 3+/-1 and 6+/-1 vs. 6+/-1 and 10+/-2% for mastoparan in the absence and presence of glibenclamide or iberiotoxin, respectively). Similar results were observed for mastoparan-7 but the inactive analogue mastoparan-17 had no effect on pial artery diameter. These data show that G protein activation elicits cerebrovasodilation through interaction with K-ATP and K-Ca channels. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:369 / 372
页数:4
相关论文
共 13 条
[1]  
AMSTEAD WM, 1996, AM J PHYSIOL, V270, pH423
[2]  
AMSTEAD WM, 1997, AM J PHYSIOL, V272, pH1785
[3]   Role of impaired cAMP and calcium-sensitive K+ channel function in altered cerebral hemodynamics following brain injury [J].
Armstead, WM .
BRAIN RESEARCH, 1997, 768 (1-2) :177-184
[4]   Hypotension dilates pial arteries by KATP and Kca channel activation [J].
Armstead, WM .
BRAIN RESEARCH, 1999, 816 (01) :158-164
[5]   Regulation of the cerebral circulation: Role of endothelium and potassium channels [J].
Faraci, FM ;
Heistad, DD .
PHYSIOLOGICAL REVIEWS, 1998, 78 (01) :53-97
[6]  
HIGASHIJIMA T, 1990, J BIOL CHEM, V265, P14176
[7]   ROLE OF ATP-SENSITIVE K+ CHANNELS IN CGRP-INDUCED DILATATION OF BASILAR ARTERY IN-VIVO [J].
KITAZONO, T ;
HEISTAD, DD ;
FARACI, FM .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (02) :H581-H585
[8]   MASTOPARAN MAY ACTIVATE GTP HYDROLYSIS BY G(I)-PROTEINS IN HL-60 MEMBRANES INDIRECTLY THROUGH INTERACTION WITH NUCLEOSIDE DIPHOSPHATE KINASE [J].
KLINKER, JF ;
HAGELUKEN, A ;
GRUNBAUM, L ;
HEILMANN, I ;
NURNBERG, B ;
HARHAMMER, R ;
OFFERMANNS, S ;
SCHWANER, I ;
ERVENS, J ;
WENZELSEIFERT, K ;
MULLER, T ;
SEIFERT, R .
BIOCHEMICAL JOURNAL, 1994, 304 :377-383
[9]   Activation of GTP formation and high-affinity GTP hydrolysis by mastoparan in various cell membranes - G-protein activation via nucleoside diphosphate kinase, a possible general mechanism of mastoparan action [J].
Klinker, JF ;
Laugwitz, KL ;
Hageluken, A ;
Seifert, R .
BIOCHEMICAL PHARMACOLOGY, 1996, 51 (03) :217-223
[10]  
Komaru T, 1997, CIRC RES, V80, P1