Rapid degradation of the Cry1F insecticidal crystal protein in soil

被引:43
作者
Herman, RA
Wolt, JD
Halliday, WR
机构
[1] Dow AgroSci LLC, Indianapolis, IN 46268 USA
[2] Battelle Mem Inst, Cleveland, OH 44135 USA
关键词
Bacillus thuringiensis; soil degradation; Cry1F; transgenic plants;
D O I
10.1021/jf025630u
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The gene for the core Cry1F insecticidal crystal protein (ICP) from Bacillus thuringiensis Berliner (Bt) has been incorporated into the genome of maize plants, Zea mays L. Plants expressing this ICP are protected from attack by various Lepidopteran pests including the European corn borer, Ostrinia nubilalis (Hubner). The stability of the Cry1F ICP in soil was assessed in a laboratory study designed to determine the persistence of the active protein residue in soil over time, using insect bioassay as the analytical quantification method. The GI(50) (concentration estimated to inhibit growth by 50%) rose at each consecutive incubation interval, indicating a consistent decline in Cry1F activity over time. The residue data were poorly described by a first-order model when fit to either the full data or a truncated data set where the last interval (28 days) was excluded. Data were well described by a shift-log model, and this model predicted DT50 (time until 50% decay) and DT90 (time until 90% decay) values of 0.6 and 6.9 days, respectively. This rapid degradation rate was consistent with other Bt proteins evaluated in our laboratory.
引用
收藏
页码:7076 / 7078
页数:3
相关论文
共 16 条
  • [1] GLARE TR, 2000, BACILLUS THURIGIENSI
  • [2] NONLINEAR PESTICIDE DISSIPATION IN SOIL - A NEW MODEL BASED ON SPATIAL VARIABILITY
    GUSTAFSON, DI
    HOLDEN, LR
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1990, 24 (07) : 1032 - 1038
  • [3] No detection of Cry1Ac protein in soil after multiple years of transgenic Bt cotton (Bollgard) use
    Head, G
    Surber, JB
    Watson, JA
    Martin, JW
    Duan, JJ
    [J]. ENVIRONMENTAL ENTOMOLOGY, 2002, 31 (01) : 30 - 36
  • [4] Rapid degradation of a binary, PS149B1, δ-endotoxin of Bacillus thuringiensis in soil, and a novel mathematical model for fitting curve-linear decay
    Herman, RA
    Scherer, PN
    Wolt, JD
    [J]. ENVIRONMENTAL ENTOMOLOGY, 2002, 31 (02) : 208 - 214
  • [5] Rapid degradation of Cry1F delta-endotoxin in soil
    Herman, RA
    Evans, SL
    Shanahan, DM
    Mihaliak, CA
    Bormett, GA
    Young, DL
    Buehrer, J
    [J]. ENVIRONMENTAL ENTOMOLOGY, 2001, 30 (04) : 642 - 644
  • [6] LARGE-SCALE REARING OF A STERILE BACKCROSS OF THE TOBACCO BUDWORM (LEPIDOPTERA, NOCTUIDAE)
    KING, EG
    HARTLEY, GG
    MARTIN, DF
    LASTER, ML
    [J]. JOURNAL OF ECONOMIC ENTOMOLOGY, 1985, 78 (05) : 1166 - 1172
  • [7] Persistence in soil of transgenic plant produced Bacillus thuringiensis var kurstaki delta-endotoxin
    Palm, CJ
    Schaller, DL
    Donegan, KK
    Seidler, RJ
    [J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1996, 42 (12) : 1258 - 1262
  • [8] QUANTIFICATION IN SOIL OF BACILLUS-THURINGIENSIS VAR KURSTAKI DELTA-ENDOTOXIN FROM TRANSGENIC PLANTS
    PALM, CJ
    DONEGAN, K
    HARRIS, D
    SEIDLER, RJ
    [J]. MOLECULAR ECOLOGY, 1994, 3 (02) : 145 - 151
  • [9] SAS Institute, 1990, SAS STAT US GUID VER
  • [10] Bt toxin is released in root exudates from 12 transgenic corn hybrids representing three transformation events
    Saxena, D
    Flores, S
    Stotzky, G
    [J]. SOIL BIOLOGY & BIOCHEMISTRY, 2002, 34 (01) : 133 - 137