Syntrophic growth of sulfate-reducing bacteria and colorless sulfur bacteria during oxygen limitation

被引:49
作者
vandenEnde, FP [1 ]
Meier, J [1 ]
vanGemerden, H [1 ]
机构
[1] UNIV GRONINGEN,DEPT MICROBIOL,NL-9751 NN HAREN,NETHERLANDS
关键词
Thiobacillus; Desulfovibrio; oxygen limitation; sulfide oxidation; sulfate reduction;
D O I
10.1016/S0168-6496(97)00014-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Stable co-cultures of the sulfate-reducing bacterium Desulfovibrio desulfuricans PA2805 and the colorless sulfur bacterium Thiobacillus thioparus T5 were obtained in continuous cultures supplied with limiting amounts of lactate and oxygen while sulfate was present in excess. Neither species could grow in purl culture under these conditions. Desulfovibrio could grow only when the oxygen concentration was kept low by Thiobacillus. Zerovalent sulfur (S-0) produced by Thiobacillus was preferred over sulfate as electron acceptor by Desulfovibrio, but the affinity for S-0 seemed to be rather low. This substrate was more efficiently used when sulfide was present suggesting that SO is preferably used in the form of polysulfides. Through the use of SO as electron acceptor the sulfide production per lactate by Desulfovibrio was four times higher than with sulfate as acceptor. Thiobacillus produces less sulfate and more S-0 when the amount of sulfide available per oxygen increases. The elevated sulfide production by Desulfovibrio thus resulted in an increase of the S-0 production by Thiobacillus, again leading to a further increase of the sulfide production. This positive feedback mechanism stabilizes the syntrophic association. The yield on lactate of Desulfovibrio was doubled in the mixed culture compared with growth on lactate and sulfate in pure culture. This yield increase was attributed to the use of zerovalent sulfur instead of sulfate as electron acceptor. Both organisms were thus shown to benefit from a syntrophic interaction in which lactate was oxidized with oxygen, with a rapid cycling of sulfide and zerovalent sulfur serving the transfer of reducing equivalents between the species. These observations shed some light on the occurrence of colorless sulfur bacteria and sulfate-reducing bacteria at the same depth horizons in microbial mats.
引用
收藏
页码:65 / 80
页数:16
相关论文
共 56 条
[1]   EFFECTS OF OXYGEN ON THE GROWTH OF DESULFOVIBRIO-DESULFURICANS [J].
ABDOLLAHI, H ;
WIMPENNY, JWT .
JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 :1025-1030
[2]   GROWTH YIELDS AND GROWTH-RATES OF DESULFOVIBRIO-VULGARIS-(MARBURG) GROWING ON HYDROGEN PLUS SULFATE AND HYDROGEN PLUS THIOSULFATE AS SOLE ENERGY-SOURCES [J].
BADZIONG, W ;
THAUER, RK .
ARCHIVES OF MICROBIOLOGY, 1978, 117 (02) :209-214
[3]   A NOVEL TYPE OF ENERGY-METABOLISM INVOLVING FERMENTATION OF INORGANIC SULFUR-COMPOUNDS [J].
BAK, F ;
CYPIONKA, H .
NATURE, 1987, 326 (6116) :891-892
[4]  
Bertolacini RJ, 1957, ANAL CHEM, V29, P281, DOI [DOI 10.1021/AC60122A031ANCHAM0003-2700, 10.1021/ac60122a031, DOI 10.1021/AC60122A031]
[5]   GROWTH OF SULFATE-REDUCING BACTERIA WITH SULFUR AS ELECTRON-ACCEPTOR [J].
BIEBL, H ;
PFENNIG, N .
ARCHIVES OF MICROBIOLOGY, 1977, 112 (01) :115-117
[6]   RAPID GAS-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF POLY-BETA-HYDROXYBUTYRIC ACID IN MICROBIAL BIOMASS [J].
BRAUNEGG, G ;
SONNLEITNER, B ;
LAFFERTY, RM .
EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1978, 6 (01) :29-37
[7]  
BUISMAN CJN, 1989, APPL MICROBIOL BIOT, V32, P363, DOI 10.1007/BF00184990
[8]   AEROBIC SULFATE REDUCTION IN MICROBIAL MATS [J].
CANFIELD, DE ;
DESMARAIS, DJ .
SCIENCE, 1991, 251 (5000) :1471-1473
[9]   SURVIVAL OF SULFATE-REDUCING BACTERIA AFTER OXYGEN STRESS, AND GROWTH IN SULFATE-FREE OXYGEN-SULFIDE GRADIENTS [J].
CYPIONKA, H ;
WIDDEL, F ;
PFENNING, N .
FEMS MICROBIOLOGY ECOLOGY, 1985, 31 (01) :39-45
[10]  
CYPIONKA H, 1994, NATO ASI SERIES G, V35, P367