Genetic characterization of dengue virus type 3 isolates in the State of Rio de Janeiro, 2001

被引：28

作者：

Miagostovich, MP ^{[1
]}

dos Santos, FB ^{[1
]}

de Simone, TS ^{[1
]}

Costa, EV ^{[1
]}

Filippis, AMB ^{[1
]}

Schatzmayr, HG ^{[1
]}

Nogueira, RMR ^{[1
]}

机构：

[1] Fiocruz MS, Inst Oswaldo Cruz, Fdn Oswaldo Cruz,Pavilhao Cardoso Fontes, Dept Virol, BR-21045900 Rio De Janeiro, RJ, Brazil

来源：

BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH | 2002年 / 35卷 / 08期

关键词：

dengue viruses type 3; sequencing; RSS-PCR; Rio de Janeiro; Brazil;

D O I：

10.1590/S0100-879X2002000800002

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures.

引用

页码：869 / 872

页数：4

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