Dynamic usage of transcription start sites within core promoters

被引：60

作者：

Kawaji, Hideya

Frith, Martin C.

Katayama, Shintaro

Sandelin, Albin

Kai, Chikatoshi

Kawai, Jun

Carninci, Piero

Hayashizaki, Yoshihide

机构：

[1] Nippon Telegraph & Tel Corp, Software Corp, Naka Ku, Yokohama, Kanagawa 2318551, Japan

[2] RIKEN, Yokohama Inst, Genome Explorat Res Grp, Genome Network Project Core Grp,Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[3] Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia

[4] Univ Copenhagen, Bioinformat Ctr, DK-2100 Copenhagen O, Denmark

[5] RIKEN, Wako Inst, Discovery Res Inst, Genome Sci Lab, Wako, Saitama 3510198, Japan

来源：

GENOME BIOLOGY | 2006年 / 7卷 / 12期

关键词：

D O I：

10.1186/gb-2006-7-12-r118

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Mammalian promoters do not initiate transcription at single, well defined base pairs, but rather at multiple, alternative start sites spread across a region. We previously characterized the static structures of transcription start site usage within promoters at the base pair level, based on large-scale sequencing of transcript 5' ends. Results: In the present study we begin to explore the internal dynamics of mammalian promoters, and demonstrate that start site selection within many mouse core promoters varies among tissues. We also show that this dynamic usage of start sites is associated with CpG islands, broad and multimodal promoter structures, and imprinting. Conclusion: Our results reveal a new level of biologic complexity within promoters - fine-scale regulation of transcription starting events at the base pair level. These events are likely to be related to epigenetic transcriptional regulation.

引用

页数：10

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