Imaging mass spectrometry: a new tool to investigate the spatial organization of peptides and proteins in mammalian tissue sections

被引：178

作者：

Chaurand, P

Schwartz, SA

Caprioli, RM

机构：

[1] Vanderbilt Univ, Mass Spectrometry Res Ctr, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Dept Biochem, Nashville, TN 37232 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2002年 / 6卷 / 05期

关键词：

D O I：

10.1016/S1367-5931(02)00370-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MALDI MS imaging mass spectrometry can be used to map the distribution of targeted compounds in tissue sections with a spatial resolution currently of about 50 mum, providing important molecular information in many areas of biological research. After matrix application, a raster of a section by the laser beam yields ions from compounds in a tissue mass-to-charge range from 1000 to over 100 000. Two-dimensional intensity maps can then be reconstructed to provide specific molecular images of a tissue.

引用

页码：676 / 681

页数：6

共 38 条

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