Treatment and sampling protocols for transgenic mutation assays

被引：36

作者：

Heddle, JA

Martus, HJ

Douglas, GR

机构：

[1] York Univ, Dept Biol, N York, ON M3J 1P3, Canada

[2] Novartis Pharma AG, Preclin Safety, ICPP, Genet Toxicol & Funct Validat, Basel, Switzerland

[3] Ctr Environm Hlth, Safe Environm Program, Mutagenesis Sect, Ottawa, ON, Canada

来源：

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 2003年 / 41卷 / 01期

关键词：

manifestation; fixation; expression; tissue specificity; neutral;

D O I：

10.1002/em.10131

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The standard protocol for testing chemicals with the transgenic mutation assays in vivo includes a period of time between treatment and sampling to permit the mutation frequency to reach a maximum. Recent evidence has shown, however, that for some chemicals the mutant frequency can decline substantially during this period, which would reduce the sensitivity of the assay. Here we discuss alternate protocols to maintain the sensitivity of the assay for both types of mutagens and, in particular, propose that treatments should continue until the time of sampling. (C) 2003 Wiley-Liss, Inc.

引用

页码：1 / 6

页数：6

共 36 条

[1] Proliferation is necessary for both repair and mutation in transgenic mouse cells [J].