Evaluation of a wipe surface sample method for collection of Bacillus spores from nonporous surfaces

被引：73

作者：

Brown, Gary S.

Betty, Rita G.

Brockmann, John E.

Lucero, Daniel A.

Souza, Caroline A.

Walsh, Kathryn S.

Boucher, Raymond M.

Tezak, Mathew

Wilson, Mollye C.

Rudolph, Todd

机构：

[1] Sandia Natl Labs, Albuquerque, NM 87185 USA

[2] Orion Int Technol, Albuquerque, NM 87185 USA

[3] Amer Staff Augmentat Providers, Albuquerque, NM 87185 USA

[4] Tact Staffing Resources, Albuquerque, NM 87185 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 03期

关键词：

D O I：

10.1128/AEM.01082-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polyester-rayon blend wipes were evaluated for efficiency of extraction and recovery of powdered Bacillus atrophaeus spores from stainless steel and painted wallboard surfaces. Method limits of detection were also estimated for both surfaces. The observed mean efficiency of polyester-rayon blend wipe recovery from stainless steel was 0.35 with a standard deviation of 0.12, and for painted wallboard it was 0.29 with a standard deviation of 0.15. Evaluation of a sonication extraction method for the polyester-rayon blend wipes produced a mean extraction efficiency of 0.93 with a standard deviation of +/- 0.09. Wipe recovery quantitative limits of detection were estimated at 90 CFU per unit of stainless steel sample area and 105 CFU per unit of painted wallboard sample area. The method recovery efficiency and limits of detection established in this work provide useful guidance for the planning of incident response environmental sampling following the release of a biological agent such as Bacillus anthracis.

引用

页码：706 / 710

页数：5

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[2]

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[3]

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