A Functional Genomics Approach to Tanshinone Biosynthesis Provides Stereochemical Insights

被引：222

作者：

Gao, Wei ^{[1
,2
]}

Hillwig, Matthew L. ^{[3
]}

Huang, Luqi ^{[1
]}

Cui, Guanghong ^{[1
]}

Wang, Xueyong ^{[1
]}

Kong, Jianqiang ^{[4
]}

Yang, Bin ^{[1
]}

Peters, Reuben J. ^{[3
]}

机构：

[1] Chinese Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

[2] Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China

[3] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA

[4] Peking Union Med Coll, Beijing 100050, Peoples R China

来源：

ORGANIC LETTERS | 2009年 / 11卷 / 22期

关键词：

ABIETADIENE SYNTHASE; (+)-COPALYL DIPHOSPHATE; ABIES-GRANDIS; MECHANISM; PLANTS;

D O I：

10.1021/ol902051v

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Tanshinones are abietane-type norditerpenoid quinone natural products that are the bioactive components of the Chinese medicinal herb Salvia miltiorrhiza Bunge. The initial results from a functional genomics-based investigation of tanshinone biosynthesis, specifically the functional identification of the relevant diterpene synthases from S. miltiorrhiza, are reported, The cyclohexa-1,4-diene arrangement of the distal ring poises the resulting miltiradiene for the ensuing aromatization and hydroxylation to ferruginol suggested for tanshinone biosynthesis.

引用

页码：5170 / 5173

页数：4

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