MEL-28/ELYS is required for the recruitment of nucleoporins to chromatin and postmitotic nuclear pore complex assembly

被引：200

作者：

Franz, Cerstin ^{[1
]}

Walczak, Rudolf ^{[1
]}

Yavuz, Sevil ^{[1
]}

Santarella, Rachel ^{[1
]}

Gentzel, Marc ^{[1
]}

Askjaer, Peter ^{[1
]}

Galy, Vincent ^{[1
]}

Hetzer, Martin ^{[1
]}

Mattaj, Iain W. ^{[1
]}

Antonin, Wolfram ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

EMBO REPORTS | 2007年 / 8卷 / 02期

关键词：

mitosis; kinetochore; nuclear envelope assembly; nuclear pore complex; Nup107-160; complex; MEL-28;

D O I：

10.1038/sj.embor.7400889

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The metazoan nuclear envelope (NE) breaks down and re-forms during each cell cycle. Nuclear pore complexes (NPCs), which allow nucleocytoplasmic transport during interphase, assemble into the re-forming NE at the end of mitosis. Using in vitro NE assembly, we show that the vertebrate homologue of MEL-28 (maternal effect lethal), a recently discovered NE component in Caenorhabditis elegans, functions in postmitotic NPC assembly. MEL-28 interacts with the Nup107-160 complex (Nup for nucleoporin), an important building block of the NPC, and is essential for the recruitment of the Nup107-160 complex to chromatin. We suggest that MEL-28 acts as a seeding point for NPC assembly.

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页码：165 / 172

页数：8

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