Cysteine-rich LIM-only proteins CRP1 and CRP2 are potent smooth muscle differentiation cofactors

被引:210
作者
Chang, DF
Belaguli, NS
Iyer, D
Roberts, WB
Wu, SP
Dong, XR
Marx, JG
Moore, MS
Beckerle, MC
Majesky, MW
Schwartz, RJ [1 ]
机构
[1] Baylor Coll Med, Ctr Cardiovasc Dev, Houston, TX 77030 USA
[2] Baylor Coll Med, Grad Program Cardiovasc Sci, Houston, TX 77030 USA
[3] Baylor Coll Med, Dept Surg, Houston, TX 77030 USA
[4] Baylor Coll Med, Dept Med, Houston, TX 77030 USA
[5] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA
[6] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA
[7] Huntsman Canc Inst, Salt Lake City, UT 84112 USA
关键词
D O I
10.1016/S1534-5807(02)00396-9
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cysteine-rich LIM-only proteins, CRP1 and CRP2, expressed during cardiovascular development act as bridging molecules that associate with serum response factor and GATA proteins. SRF-CRP-GATA complexes strongly activated smooth muscle gene targets. CRP2 was found in the nucleus during early stages of coronary smooth muscle differentiation from proepicardial cells. A dominant-negative CRP2 mutant blocked proepicardial cells from differentiating into smooth muscle cells. Together with SRIF and GATA proteins, CRP1 and CRP2 converted pluripotent 10T1/2 fibroblasts into smooth muscle cells, while muscle LIM protein CRP3 inhibited the conversion. Thus, LIM-only proteins of the CRP family play important roles in organizing multiprotein complexes, both in the cytoplasm, where they participate in cytoskeletal remodeling, and in the nucleus, where they strongly facilitate smooth muscle differentiation.
引用
收藏
页码:107 / 118
页数:12
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