Absorption of kininogen from human plasma by Streptococcus pyogenes is followed by the release of bradykinin

被引:45
作者
BenNasr, A
Herwald, H
Sjobring, U
Renne, T
MullerEsterl, W
Bjorck, L
机构
[1] LUND UNIV, DEPT CELL & MOL BIOL, SECT MOL PATHOGENESIS, S-22100 LUND, SWEDEN
[2] LUND UNIV, DEPT MED MICROBIOL, S-22362 LUND, SWEDEN
[3] UNIV MAINZ, INST PHYSIOL CHEM & PATHOBIOCHEM, D-55099 MAINZ, GERMANY
关键词
D O I
10.1042/bj3260657
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
H-kininogen (high-molecular-mass kininogen, HK) is the precursor of the vasoactive peptide hormone bradykinin (BK). Previous work has demonstrated that HK binds to Streptococcus pyrogenes through M-proteins, fibrous surface proteins and important virulence factors of these bacteria. Here we find that M-protein-expressing bacteria absorb HK from human plasma. The HK bound to the bacteria was found to be cleaved, and analysis of the degradation pattern suggested that the cleavage of HK at the bacterial surface is associated with the release of BK. Moreover, addition of activated plasma prekallikrein to bacteria preincubated with human plasma, resulted in BK release. This mechanism, by which a potent vasoactive and proinflammatory peptide is generated at the site of infection, should influence the host-parasite relationship during S. pyogenes infections.
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页码:657 / 660
页数:4
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