Optical induction of synaptic plasticity using a light-sensitive channel

被引：178

作者：

Zhang, Yan-Ping ^{[1
]}

Oertner, Thomas G. ^{[1
]}

机构：

[1] Novartis Res Fdn, Friedrich Miescher Inst, CH-4058 Basel, Switzerland

来源：

NATURE METHODS | 2007年 / 4卷 / 02期

关键词：

D O I：

10.1038/nmeth988

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have combined millisecond activation of channelrhodopsin-2 (ChR2), a light-gated ion channel, with two-photon calcium imaging to investigate active synaptic contacts in rat hippocampal slice cultures. Calcium influx was larger during light-induced action potentials than during action potentials induced by somatic current injection, leading to highly reproducible synaptic transmission. Pairing of light stimulation with postsynaptic depolarization induced long-term potentiation, making this technique ideal for genetic and pharmacological dissection of synaptic plasticity.

引用

页码：139 / 141

页数：3

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