Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides

被引:28
作者
Bernhard, Oliver K.
Kapp, Eugene A.
Simpson, Richard J.
机构
[1] Royal Melbourne Hosp, Joint Proteom Lab, Ludwig Inst Canc Res, Parkville, Vic 3050, Australia
[2] Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Parkville, Vic 3050, Australia
关键词
N-glycosylation; cysteine-containing peptides; mouse plasma; mass spectrometry; proteomics; human plasma proteome;
D O I
10.1021/pr0604559
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A comprehensive understanding of the mouse plasma proteome is important for studies using mouse models to identify protein markers of human disease. To enhance our analysis of the mouse plasma proteome, we have developed a method for isolating low-abundance proteins using a cysteine-containing glycopeptide strategy. This method involves two orthogonal affinity capture steps. First, glycoproteins are coupled to an azlactone copolymer gel using hydrazide chemistry and cysteine residues are then biotinylated. After trypsinization and extensive washing, tethered N-glycosylated tryptic peptides are released from the gel using PNGase F. Biotinylated cysteinyl-containing glycopeptides are then affinity selected using a monomeric avidin gel and analyzed by LC-MS/MS. We have applied the method to a proteome analysis of mouse plasma. In two independent analyses using 200 mu L each of C57BL mouse plasma, 51 proteins were detected. Only 42 proteins were seen when the same plasma sample was analyzed by glycopeptides only. A total of 104 N-glycosylation sites were identified. Of these, 17 sites have hitherto not been annotated in the Swiss-Prot database whereas 48 were considered probable, potential, or by similarity - i.e., based on little or no experimental evidence. We show that analysis by cysteine-containing glycopeptides allows detection of low-abundance proteins such as the epidermal growth factor receptor, the Vitamin K-dependent protein Z, the hepatocyte growth factor activator, and the lymphatic endothelium-specific hyaluronan receptor as these proteins were not detected in the glycopeptide control analysis.
引用
收藏
页码:987 / 995
页数:9
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