A protective role for the polyamine spermine against drought stress in Arabidopsis

被引:232
作者
Yamaguchi, Koji
Takahashi, Yoshihiro
Berberich, Thomas
Imai, Akihiko
Takahashi, Taku
Michael, Anthonv J.
Kusano, Tomonobu [1 ]
机构
[1] Tohoku Univ, Grad Sch Life Sci, Aoba Ku, Sendai, Miyagi 9808577, Japan
[2] Okayama Univ, Grad Sch Nat Sci & Technol, Div Biosci, Okayama 7008530, Japan
[3] Inst Food Res, Inst Food Res, Norwich NR4 7UA, Norfolk, England
关键词
Arabidopsis thaliana; calcium homeostasis; drought-stress; polyamine; spermine; stomata;
D O I
10.1016/j.bbrc.2006.11.041
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cellular polyamine content often changes in response to abiotic stresses. However, its physiological relevance is unknown. We found that an Arabidopsis mutant plant (acl5lspms), which cannot produce spermine, is hypersensitive to high salt. Examination of drought sensitivity of the mutant and comparison with wild type plants indicated hypersensitivity to drought. This phenotype was cured by spermine pretreatment but not by the other polyamines putrescine and spermidine, suggesting that drought-hypersensitivity exhibited by the mutant is due to spermine deficiency. The water loss rate of wild type and mutant plants were similar until 20 min after onset of dehydration stress, but after a longer exposure the rate in mutant plants was higher than in wild type plants. Consistent with this result, the stomata of the mutant leaves remained open while in wild type leaves they closed. Based on the collected data, we discuss a role for spermine in response to drought stress. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:486 / 490
页数:5
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