Phylogenetic analysis of Phlebotomus species belonging to the subgenus Larroussius (Diptera, Psychodidae) by ITS2 rDNA sequences

被引:44
作者
Di Muccio, T
Marinucci, M
Frusteri, L
Maroli, M
Pesson, B
Gramiccia, M
机构
[1] Ist Super Sanita, Lab Parassitol, I-00161 Rome, Italy
[2] Univ Strasbourg 1, Fac Pharm, Parasitol Lab, F-67070 Strasbourg, France
关键词
Phlebotomus; Larroussius; Leishmania infantum; rDNA; ITS2; phylogeny;
D O I
10.1016/S0965-1748(00)00012-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the genealogy of Phlebotomus (Diptera: Psychodidae), morphological analyses have indicated that the subgenus Larroussius is a monophyletic group which is most closely related to the subgenera Transphlebotomus and Adlerius. We conducted a phylogenetic analysis of the relationships among six representative species of the subgenus Larroussius and one species representatitive of the Phlebotomus subgenus, assessing sequences of the Second Internal Transcribed Spacer (ITS2) of the ribosomal RNA (rRNA). Three of the species (P. perniciosus, P. ariasi and P. perfiliewi perfiliewi) were collected in different parts of the Mediterranean area. The trees estimated from parsimony and neighbour-joining analyses supported the monophyly of the Larroussius subgenus inferred from the morphological analysis. According to our data, P. ariasi may be a sister group to the rest of the Larroussius subgenus, although additional sequence data are needed to confirm this observation. Our results suggest that P. perniciosus and P. longicuspis are distinct species, in spite of the fact that there are only slight morphological differences. The strict congruence between the phylogeny of the Larroussius subgenus inferred from the ITS2 sequences and that based on morphological studies further confirmed the ability of the spacer sequence to identify recently-derived affiliations. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:387 / 393
页数:7
相关论文
共 28 条
[1]  
Artemiev M.M., 1991, Parassitologia (Rome), V33, P69
[2]  
Ashford R.W., 1991, Parassitologia (Rome), V33, P79
[3]   Morphological and isoenzymatic differentiation of sympatric populations of Phlebotomus perniciosus and Phlebotomus longicuspis (Diptera: Psychodidae) in northern Morocco [J].
Benabdennbi, I ;
Pesson, B ;
Cadi-Soussi, M ;
Marquez, FM .
JOURNAL OF MEDICAL ENTOMOLOGY, 1999, 36 (01) :116-120
[4]  
Collantes F., 1997, Boletin de la Asociacion Espanola de Entomologia, V21, P141
[5]   A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species [J].
Collins, FH ;
Paskewitz, SM .
INSECT MOLECULAR BIOLOGY, 1996, 5 (01) :1-9
[6]   Molecular systematics of Phlebotominae: a pilot study. Paraphyly of the genus Phlebotomus [J].
Depaquit, J ;
Perrotey, S ;
Lecointre, G ;
Tillier, A ;
Tillier, S ;
Ferte, H ;
Kaltenbach, M ;
Leger, N .
COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES, 1998, 321 (10) :849-855
[7]   Mitochondrial haplotypes and phylogeography of Phlebotomus vectors of Leishmania major [J].
Esseghir, S ;
Ready, PD ;
KillickKendrick, R ;
BenIsmail, R .
INSECT MOLECULAR BIOLOGY, 1997, 6 (03) :211-225
[8]  
FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
[9]   PHLEBOTOMINE VECTORS OF THE LEISHMANIASES - A REVIEW [J].
KILLICKKENDRICK, R .
MEDICAL AND VETERINARY ENTOMOLOGY, 1990, 4 (01) :1-24