A label-free electrochemical immunoassay for carcinoembryonic antigen (CEA) based on gold nanoparticles (AuNPs) and nonconductive polymer film

被引：143

作者：

Tang, Hao

Chen, Jinhua ^{[1
]}

Nie, Lihua

Kuang, Yafei

Yao, Shouzhuo

机构：

[1] Hunan Univ, State Key Lab Chem Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Peoples R China

[2] Hunan Normal Univ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 06期

基金：

中国国家自然科学基金;

关键词：

gold nanoparticles; poly-o-aminophenol film; electrochemical impedance spectroscopy; immunoassay; carcinoembryonic antigen;

D O I：

10.1016/j.bios.2006.04.027

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A simple and sensitive label-free electrochemical immunoassay electrode for detection of carcinoembryonic antigen (CEA) has been developed. CEA antibody (CEAAb) was covalently attached on glutathione (GSH) monolayer-modified gold nanoparticle (AuNP) and the resulting CEAAb-AuNP bioconjugates were immobilized on Au electrode by electro-copolymerization with o-aminophenol (OAP). Electrochemical impedance spectroscopy and cyclic voltammetry studies demonstrate that the formation of CEA antibody-antigen complexes increases the electron transfer resistance of [Fe(CN)(6)](3-/4-) redox pair at the poly-OAP/CEAAb-AuNP/Au electrode. The use of CEA antibody-AuNP bioconjugates and poly-OAP film could enhance the sensitivity and anti-non specific binding of the resulting immunoassay electrode. The preliminary application of poly-OAP/CEAAb-AuNP/Au electrode for detection of CEA was also evaluated. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：1061 / 1067

页数：7

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