Separation of IgG from human plasma using thiophilic hollow fiber membranes

被引:20
作者
Coffinier, Y
Legallais, C
Vijayalakshmi, MA
机构
[1] Univ Technol Compiegne, Ctr Rech Royallieu, LIMTechS, F-60205 Compiegne, France
[2] Univ Technol Compiegne, Ctr Rech Royallieu, Lab Biomecan & Genie Biomed, F-60205 Compiegne, France
关键词
thiophilic chromatography; poly(ethylenevinylalcohol) hollow fiber; affinity membrane; immunoglobulins G; human plasma;
D O I
10.1016/S0376-7388(02)00132-1
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
To replace conventional bioaffinity ligand like protein A or protein G, pseudobioaffinity ligands seem to be an interesting alternative for the purification of IgG. In this study, thiophilic ligand (T-gel) was immobilized on a copolymer of ethylene and vinyl alcohol (PEVA) based hollow fiber membrane to obtain an affinity support for the purification of IgG from human plasma. Microporous hollow fibers have been modified via divinyl sulfone activation and coupled by 2-mercaptoethanol (T-PEVA). The affinity module allowed the purification of IgG from human plasma with high efficiency (85% of IgG loaded were adsorbed) with a very small contamination of albumin (<1%). The enrichment was quite high (IgG/albumin) as shown by a final ratio of 39.3/0.27 before loading. The binding capacity could allow the separation of 1.5 g of IgG from human plasma with a functionalized membrane surface area of 1 m(2). We tried to minimize sample preparation by decreasing the salt concentration, the sample dilution and by optimizing the rinsing and elution steps. This thiophilic derivatized hollow fiber module proved that it could be used as a very efficient tool for the purification of IgG provided that high concentration of salt was added. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:13 / 22
页数:10
相关论文
共 27 条
[1]   A ONE-STEP PURIFICATION METHOD FOR MONOCLONAL-ANTIBODIES BASED ON SALT-PROMOTED ADSORPTION CHROMATOGRAPHY ON A THIOPHILIC ADSORBENT [J].
BELEW, M ;
JUNTTI, N ;
LARSSON, A ;
PORATH, J .
JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 102 (02) :173-182
[2]   The use of thiophilic chromatography for antibody purification: a review [J].
Boschetti, E .
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2001, 49 (1-3) :361-389
[3]  
BOSCHETTI E, 1999, HYDROPHOBIC CHARGE I, P1
[4]   MEMBRANE-BASED AFFINITY TECHNOLOGY FOR COMMERCIAL SCALE PURIFICATIONS [J].
BRANDT, S ;
GOFFE, RA ;
KESSLER, SB ;
OCONNOR, JL ;
ZALE, SE .
BIO-TECHNOLOGY, 1988, 6 (07) :779-782
[5]   Experimental kinetic aspects of hollow fiber membrane-based pseudobioaffinity filtration: Process for IgG separation from human plasma [J].
Bueno, SMA ;
Legallais, C ;
Haupt, K ;
Vijayalakshmi, MA .
JOURNAL OF MEMBRANE SCIENCE, 1996, 117 (1-2) :45-56
[6]   IN-VITRO REMOVAL OF HUMAN-IGG BY PSEUDOBIOSPECIFIC AFFINITY MEMBRANE FILTRATION ON A LARGE-SCALE - A PRELIMINARY-REPORT [J].
BUENO, SMA ;
HAUPT, K ;
VIJAYALAKSHMI, MA .
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, 1995, 18 (07) :392-398
[7]   SEPARATION OF IMMUNOGLOBULIN-G FROM HUMAN SERUM BY PSEUDOBIOAFFINITY CHROMATOGRAPHY USING IMMOBILIZED L-HISTIDINE IN HOLLOW-FIBER MEMBRANES [J].
BUENO, SMA ;
HAUPT, K ;
VIJAYALAKSHMI, MA .
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1995, 667 (01) :57-67
[8]   Hydrophobic charge induction chromatography: salt independent protein adsorption and facile elution With aqueous buffers [J].
Burton, SC ;
Harding, DRK .
JOURNAL OF CHROMATOGRAPHY A, 1998, 814 (1-2) :71-81
[9]  
Charcosset C, 1998, J CHEM TECHNOL BIOT, V71, P95, DOI 10.1002/(SICI)1097-4660(199802)71:2<95::AID-JCTB823>3.0.CO
[10]  
2-J