A single dose immunization with rabbit haemorrhagic disease virus major capsid protein produced in Saccharomyces cerevisiae induces protection

被引：43

作者：

Boga, JA ^{[1
]}

Alonso, JMM ^{[1
]}

Casais, R ^{[1
]}

Parra, F ^{[1
]}

机构：

[1] UNIV OVIEDO, DEPT BIOQUIM & BIOL MOL,FAC MED, INST UNIV BIOTECNOL ASTURIAS,CSIC, E-33006 OVIEDO, SPAIN

来源：

JOURNAL OF GENERAL VIROLOGY | 1997年 / 78卷

关键词：

D O I：

10.1099/0022-1317-78-9-2315

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene coding for the major capsid protein (VP60) from rabbit haemorrhagic disease virus was expressed in Saccharomyces cerevisiae under the phosphoglycerate kinase promoter. The recombinant VP60 produced in yeast was antigenically similar to the viral polypeptide as determined with a polyclonal serum. Electron microscopic observation of the recombinant yeast-derived antigen revealed the presence of virus-like particles similar in size and appearance to native capsids. Subcutaneous vaccination of rabbits with a single dose of this antigen in the absence of commercial adjuvants conferred complete protection against the haemorrhagic disease.

引用

页码：2315 / 2318

页数：4

共 17 条

[1]

Alonso JMM, 1996, J VIROL, V70, P1261

[2]

Arguello Villares JL, 1991, REV SCI TECH OIE, V10, P471

[3] MOLECULAR-CLONING, SEQUENCING AND EXPRESSION IN ESCHERICHIA-COLI OF THE CAPSID PROTEIN GENE FROM RABBIT HEMORRHAGIC-DISEASE VIRUS (SPANISH ISOLATE AST/89) [J].