Dynamic changes in histone acetylation regulate origins of DNA replication

被引：142

作者：

Unnikrishnan, Ashwin ^{[1
,2
,3
]}

Gafken, Philip R. ^{[4
]}

Tsukiyama, Toshio ^{[1
]}

机构：

[1] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98104 USA

[2] Fred Hutchinson Canc Res Ctr, Mol & Cellular Biol Program, Seattle, WA 98104 USA

[3] Univ Washington, Seattle, WA 98195 USA

[4] Fred Hutchinson Canc Res Ctr, Prote Facil, Seattle, WA 98104 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2010年 / 17卷 / 04期

基金：

美国国家卫生研究院;

关键词：

SACCHAROMYCES-CEREVISIAE; IN-VIVO; ACETYLTRANSFERASE HBO1; CHROMATIN-STRUCTURE; CHROMOSOMAL DNA; CELL-CYCLE; YEAST; NUCLEOSOME; METHYLATION; H4;

D O I：

10.1038/nsmb.1780

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although histone modifications have been implicated in many DNA-dependent processes, their precise role in DNA replication remains largely unknown. Here we describe an efficient single-step method to specifically purify histones located around an origin of replication from Saccharomyces cerevisiae. Using high-resolution MS, we have obtained a comprehensive view of the histone modifications surrounding the origin of replication throughout the cell cycle. We have discovered that acetylation of histone H3 and H4 is dynamically regulated around an origin of replication, at the level of multiply acetylated histones. Furthermore, we find that this acetylation is required for efficient origin activation during S phase.

引用

页码：430 / U68

页数：10

共 60 条

[1] Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit [J].