Combinative method using HPLC fingerprint and quantitative analyses for quality consistency evaluation of an herbal medicinal preparation produced by different manufacturers

被引：111

作者：

Li, Yan ^{[2
]}

Wu, Tao ^{[3
]}

Zhu, Jinghui ^{[2
]}

Wan, Lili ^{[2
]}

Yu, Qi ^{[2
]}

Li, Xingxia ^{[2
]}

Cheng, Zhihong ^{[1
,3
]}

Guo, Cheng ^{[2
]}

机构：

[1] Fudan Univ, Dept Pharmacognosy, Shanghai 201203, Peoples R China

[2] Shanghai Jiao Tong Univ, Dept Pharm, Shanghai Peoples Hosp 6, Shanghai 200233, Peoples R China

[3] Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai Key Lab Compound Chinese Med, Key Lab Standardizat Chinese Med,Minist Educ, Shanghai 201203, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2010年 / 52卷 / 04期

关键词：

Quality consistency; Yiqing preparation; HPLC fingerprint; Quantitative determination; Herbal preparation; PERFORMANCE LIQUID-CHROMATOGRAPHY; XIE-XIN-TANG; DIODE-ARRAY; QUANTIFICATION; COMPONENTS; PILLS;

D O I：

10.1016/j.jpba.2010.01.018

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

A combinative method using HPLC-DAD fingerprint and quantitative analysis was developed and validated for manufacturer-to-manufacturer quality consistency evaluation of Yiqing preparations. For fingerprint analysis, 22 peaks were selected as the characteristic peaks to evaluate the similarities of different samples collected from different manufacturers. The similarities of 12 Yiqing samples were beyond 0.90, indicating that samples from different manufacturers were, to some extent, consistent. Additionally, simultaneous quantification of nine markers including berberine, aloe-emodin, rhein, emodin, chrysophanol, baicalin, baicalein, wogonoside, and wogonin in Yiqing was performed to interpret the quality consistency. The results from the quantitative data showed that the contents of these nine marker compounds were quite consistent for batches produced within one manufacturer and significantly different from manufacturer-to-manufacturer. This study demonstrated that a combination of the chromatographic fingerprint and quantitative analysis offers an efficient way to quality consistency evaluation of herbal preparation. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：597 / 602

页数：6

共 22 条

[1]

[Anonymous], 2004, GUID IND BOT DRUG PR

[2]

Analysis of major alkaloids in Rhizoma coptidis by capillary electrophoresis-electrospray-time of flight mass spectrometry with different background electrolytes [J].