Structure of the cytoplasmic β subunit -: T1 assembly of voltage-dependent K+ channels

被引：271

作者：

Gulbis, JM

Zhou, M

Mann, S

MacKinnon, R

机构：

[1] Rockefeller Univ, Howard Hughes Med Inst, New York, NY 10021 USA

[2] Rockefeller Univ, Lab Mol Neurobiol & Biophys, New York, NY 10021 USA

来源：

SCIENCE | 2000年 / 289卷 / 5476期

关键词：

D O I：

10.1126/science.289.5476.123

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The structure of the cytoplasmic assembly of voltage-dependent K+ channels was solved by x-ray crystallography at 2.1 angstrom resolution. The assembly includes the cytoplasmic (T1) domain of the integral membrane or subunit together with the oxidoreductase beta subunit in a fourfold symmetric T1(4)beta(4) complex. An electrophysiological assay showed that this complex is oriented with four T1 domains facing the transmembrane pore and four beta subunits facing the cytoplasm. The transmembrane pore communicates with the cytoplasm through lateral, negatively charged openings above the T1(4)beta(4) complex. The inactivation peptides of voltage-dependent K+ channels reach their site of action by entering these openings.

引用

页码：123 / 127

页数：5

共 33 条

[1] INTERACTION OF TETRAETHYLAMMONIUM ION DERIVATIVES WITH POTASSIUM CHANNELS OF GIANT AXONS [J].