Localization and induction by dehydration of ClC-K chloride channels in the rat kidney

被引:112
作者
Vandewalle, A [1 ]
Cluzeaud, F [1 ]
Bens, M [1 ]
Kieferle, S [1 ]
Steinmeyer, K [1 ]
Jentsch, TJ [1 ]
机构
[1] UNIV HAMBURG, ZENTRUM MOL NEUROBIOL, D-20246 HAMBURG, GERMANY
关键词
immunocytochemistry;
D O I
10.1152/ajprenal.1997.272.5.F678
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We investigate the intrarenal expression of two recently cloned chloride channels, rClC-K1 and rClC-K2, by reverse transcriptase-polymerase chain reaction on single microdissected tubules from the rat kidney and by immunohistochemistry using a polyclonal antibody that recognizes both highly homologous channels. Both rClC-K1 and rClC-K2 mRNAs were detected in outer medullary late proximal tubules (S3), papillary ascending thin limbs (ATL), and outer medullary (MTAL) and cortical (CTAL) thick ascending limbs, distal tubules (DCT), and cortical, outer medullary, and inner medullary collecting ducts. Indirect immunofluorescence studies demonstrated that the rClC-K proteins were restricted to the basolateral membranes from ATL, DCT, and collecting ducts cells, whereas CTAL and MTAL exhibited a more diffuse basal staining. When rats were dehydrated, a condition which increased the expression of rClC-K1 in cortex and medulla, a weak cytoplasmic staining was found in late proximal tubule cells. Thus these results demonstrate that rat kidney ClC-K channels are predominantly located in the basolateral membranes from cells of the late segments of the renal tubule where most of chloride reabsorption takes place.
引用
收藏
页码:F678 / F688
页数:11
相关论文
共 34 条
[1]  
ADACHI S, 1994, J BIOL CHEM, V269, P17677
[2]   REGULATION OF PLASMA-MEMBRANE RECYCLING BY CFTR [J].
BRADBURY, NA ;
JILLING, T ;
BERTA, G ;
SORSCHER, EJ ;
BRIDGES, RJ ;
KIRK, KL .
SCIENCE, 1992, 256 (5056) :530-532
[3]   ClC-6 and ClC-7 are two novel broadly expressed members of the CLC chloride channel family [J].
Brandt, S ;
Jentsch, TJ .
FEBS LETTERS, 1995, 377 (01) :15-20
[4]  
FONG P, 1995, J MEMBRANE BIOL, V144, P189
[5]  
FRIZZELL RA, 1979, AM J PHYSIOL, V236, pF1
[6]   PHORBOL ESTER INDUCES THE BIOSYNTHESIS OF GLYCOSYLATED AND NONGLYCOSYLATED PLASMINOGEN-ACTIVATOR INHIBITOR-2 IN HIGH EXCESS OVER UROKINASE-TYPE PLASMINOGEN-ACTIVATOR IN HUMAN U-937 LYMPHOMA-CELLS [J].
GENTON, C ;
KRUITHOF, EKO ;
SCHLEUNING, WD .
JOURNAL OF CELL BIOLOGY, 1987, 104 (03) :705-712
[8]   A SMALL-CONDUCTANCE CL- CHANNEL IN THE MOUSE THICK ASCENDING LIMB THAT IS ACTIVATED BY ATP AND PROTEIN-KINASE A [J].
GUINAMARD, R ;
CHRAIBI, A ;
TEULON, J .
JOURNAL OF PHYSIOLOGY-LONDON, 1995, 485 (01) :97-112
[9]   LIGHT-MICROSCOPIC LECTIN HISTOCHEMISTRY IN AGING MOUSE KIDNEY - STUDY OF COMPOSITIONAL CHANGES IN GLYCOCONJUGATES [J].
HANAI, T ;
USUDA, N ;
MORITA, T ;
NAGATA, T .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1994, 42 (07) :897-906