Monomeric and oligomeric complexes of the B cell antigen receptor

被引：222

作者：

Schamel, WWA

Reth, M

机构：

[1] Univ Freiburg, Dept Mol Immunol Biol 3, D-79108 Freiburg, Germany

[2] Max Planck Inst Immunobiol, D-79108 Freiburg, Germany

来源：

IMMUNITY | 2000年 / 13卷 / 01期

关键词：

D O I：

10.1016/S1074-7613(00)00003-0

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The current structural model of the B cell antigen receptor (BCR) describes it as a symmetric protein complex in which one membrane-bound immunoglobulin molecule (mlg) is noncovalently bound on each side by an Ig-alpha/Ig-beta heterodimer. Using peptide-tagged Ig-alpha proteins, blue native polyacrylamide gel electrophoresis (BN-PAGE), and biosynthetical labeling of B cells, we find that the mIg:Ig-alpha/Ig-beta complex has a stoichiometry of 1:1 and not 1:2. An anti-flag stimulation of B cells coexpressing Flag-tagged and wild-type Ig-alpha proteins results in the phosphorylation of both Ig-alpha proteins, suggesting that on the surface of living B cells, several BCR monomers are in contact with each other. A BN-PAGE analysis after limited detergent lysis provides further evidence for an oligomeric BCR structure.

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页码：5 / 14

页数：10

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