High resolution of oligosaccharide mixtures by ultrahigh voltage micellar electrokinetic capillary chromatography

被引：25

作者：

Hutterer, KM

Birrell, H

Camilleri, P

Jorgenson, JW

机构：

[1] SmithKline Beecham Pharmaceut, New Frontiers Sci Pk, Harlow CM19 5AW, Essex, England

[2] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 2000年 / 745卷 / 02期

关键词：

oligosaccharides;

D O I：

10.1016/S0378-4347(00)00301-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligosaccharide mixtures released from ribonuclease B and human IgG have been separated using micellar electrokinetic capillary chromatography operated at 100 kV; The resolution of these closely related analytes at this high voltage was found to be superior to that obtained at 20 kV, a voltage which is ordinarily used in most capillary electrophoresis separations. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：365 / 372

页数：8

相关论文

共 9 条

[1] A dual-detection strategy in the chromatographic analysis of 2-aminoacridone-derivatized oligosaccharides [J].

Birrell, H ;

Charlwood, J ;

Lynch, I ;

North, S ;

Camilleri, P .

ANALYTICAL CHEMISTRY, 1999, 71 (01) :102-108

[2] HIGH-RESOLUTION AND RAPID ANALYSIS OF BRANCHED OLIGOSACCHARIDES BY CAPILLARY ELECTROPHORESIS [J].

CAMILLERI, P ;

HARLAND, GB ;

OKAFO, G .

ANALYTICAL BIOCHEMISTRY, 1995, 230 (01) :115-122

[3] Efficient carbohydrate release, purification, and derivatization [J].

Charlwood, J ;

Birrell, H ;

Camilleri, P .

ANALYTICAL BIOCHEMISTRY, 1998, 262 (02) :197-200

[4] Glycobiology: Toward understanding the function of sugars [J].

Dwek, RA .

CHEMICAL REVIEWS, 1996, 96 (02) :683-720

[5]

FURUKAWA K, 1991, MOL IMMUNOL, V28, P1333

[6] HOW N-LINKED OLIGOSACCHARIDES AFFECT GLYCOPROTEIN FOLDING IN THE ENDOPLASMIC-RETICULUM [J].

HELENIUS, A .

MOLECULAR BIOLOGY OF THE CELL, 1994, 5 (03) :253-265

[7] Ultrahigh-voltage capillary zone electrophoresis [J].

Hutterer, KM ;

Jorgenson, JW .

ANALYTICAL CHEMISTRY, 1999, 71 (07) :1293-1297

[8] A coordinated high-performance liquid chromatographic, capillary electrophoretic, and mass spectrometric approach for the analysis of oligosaccharide mixtures derivatized with 2-aminoacridone [J].

Okafo, G ;

Burrow, L ;

Carr, SA ;

Roberts, GD ;

Johnson, W ;

Camilleri, P .

ANALYTICAL CHEMISTRY, 1996, 68 (24) :4424-4430

[9] BIOLOGICAL ROLES OF OLIGOSACCHARIDES - ALL OF THE THEORIES ARE CORRECT [J].

VARKI, A .

GLYCOBIOLOGY, 1993, 3 (02) :97-130