What is flux balance analysis?

被引：2572

作者：

Orth, Jeffrey D. ^{[1
]}

Thiele, Ines ^{[2
]}

Palsson, Bernhard O. ^{[1
]}

机构：

[1] Univ Calif San Diego, La Jolla, CA 92093 USA

[2] Univ Iceland, Reykjavik, Iceland

来源：

NATURE BIOTECHNOLOGY | 2010年 / 28卷 / 03期

基金：

美国国家卫生研究院;

关键词：

SCALE METABOLIC RECONSTRUCTIONS; ESCHERICHIA-COLI; IN-SILICO; MODELS; NETWORKS; OPTIMIZATION; CAPABILITIES; INFORMATION;

D O I：

10.1038/nbt.1614

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Flux balance analysis is a mathematical approach for analyzing the flow of metabolites through a metabolic network. This primer covers the theoretical basis of the approach, several practical examples and a software toolbox for performing the calculations.

引用

收藏

页码：245 / 248

页数：4

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[1] Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox [J].

Becker, Scott A. ;

Feist, Adam M. ;

Mo, Monica L. ;

Hannum, Gregory ;

Palsson, Bernhard O. ;

Herrgard, Markus J. .

NATURE PROTOCOLS, 2007, 2 (03) :727-738

[2] OptKnock: A bilevel programming framework for identifying gene knockout strategies for microbial strain optimization [J].

Burgard, AP ;

Pharkya, P ;

Maranas, CD .

BIOTECHNOLOGY AND BIOENGINEERING, 2003, 84 (06) :647-657

[3] Metabolic modeling of microbial strains in silico [J].

Covert, MW ;

Schilling, CH ;

Famili, I ;

Edwards, JS ;

Goryanin, II ;

Selkov, E ;

Palsson, BO .

TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (03) :179-186

[4] Global reconstruction of the human metabolic network based on genomic and bibliomic data [J].

Duarte, Natalie C. ;

Becker, Scott A. ;

Jamshidi, Neema ;

Thiele, Ines ;

Mo, Monica L. ;

Vo, Thuy D. ;

Srivas, Rohith ;

Palsson, Bernhard O. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (06) :1777-1782

[5] Genome-scale models of bacterial metabolism: reconstruction and applications [J].

Durot, Maxime ;

Bourguignon, Pierre-Yves ;

Schachter, Vincent .

FEMS MICROBIOLOGY REVIEWS, 2009, 33 (01) :164-190

[6] Robustness analysis of the Escherichia coli metabolic network [J].

Edwards, JS ;

Palsson, BO .

BIOTECHNOLOGY PROGRESS, 2000, 16 (06) :927-939

[7] Metabolic modelling of microbes: the flux-balance approach [J].

Edwards, JS ;

Covert, M ;

Palsson, B .

ENVIRONMENTAL MICROBIOLOGY, 2002, 4 (03) :133-140

[8] Characterizing the metabolic phenotype: A phenotype phase plane analysis [J].

Edwards, JS ;

Ramakrishna, R ;

Palsson, BO .

BIOTECHNOLOGY AND BIOENGINEERING, 2002, 77 (01) :27-36

[9] In silico predictions of Escherichia coli metabolic capabilities are consistent with experimental data [J].

Edwards, JS ;

Ibarra, RU ;

Palsson, BO .

NATURE BIOTECHNOLOGY, 2001, 19 (02) :125-130

[10] The growing scope of applications of genome-scale metabolic reconstructions using Escherichia coli [J].

Feist, Adam M. ;

Palsson, Bernhard O. .

NATURE BIOTECHNOLOGY, 2008, 26 (06) :659-667