A proposal for a standardised protocol to barcode all land plants

被引：690

作者：

Chase, Mark W.

Cowan, Robyn S.

Hollingsworth, Peter M.

van den Berg, Cassio

Madrinan, Santiago

Petersen, Gitte

Seberg, Ole

Jorgsensen, Tina

Cameron, Kenneth M.

Carine, Mark

Pedersen, Niklas

Hedderson, Terry A. J.

Conrad, Ferozah

Salazar, Gerardo A.

Richardson, James E.

Hollingsworth, Michelle L.

Barraclough, Timothy G.

Kelly, Laura

Wilkinson, Mike

机构：

[1] Royal Bot Gardens, Edinburgh EH3 5LR, Midlothian, Scotland

[2] Univ Los Andes, Dept Ciencias Biol, Bogota, Colombia

[3] Nat Hist Museum, Copenhagen, Denmark

[4] New York Bot Garden, Lewis B & Dorothy Cullman Program Mol Syst Studie, Bronx, NY 10458 USA

[5] Nat Hist Museum, Dept Bot, London SW7 5BD, England

[6] Univ Cape Town, Bolus Herbarium, Dept Bot, ZA-7701 Rondebosch, South Africa

[7] Kirstenbosch Res Ctr, Leslie Hill Mol Syst Lab, ZA-7735 Cape Town, South Africa

[8] Univ Nacl Autonoma Mexico, Inst Biol, Dept Bot, Mexico City 04510, DF, Mexico

[9] Imperial Coll London, Royal Bot Gardens, Div Biol, Ascot SL5 7PY, Berks, England

[10] Univ Wales, Inst Biol Sci, Aberystwyth SY23 3DA, Dyfed, Wales

来源：

TAXON | 2007年 / 56卷 / 02期

关键词：

ITS nrDNA; land plant barcoding; matK; mitochondrial DNA; plastid DNA; psbA-trnH; rbcL; rpoC1; rpoB; trnL;

D O I：

10.1002/tax.562004

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

We propose in this paper to use three regions of plastid DNA as a standard protocol for barcoding all land plants. We review the other markers that have been proposed and discuss their advantages and disadvantages. The low levels of variation in plastid DNA make three regions necessary; there are no plastid regions, coding or non-coding, that evolve as rapidly as mitochondrial DNA generally does in animals. We outline two, three-region options, (1) rpoCl, rpoB and matK or (2) rpoCl, malK and psbA-trnH as viable markers for land plant barcoding.

引用

页码：295 / 299

页数：5

共 17 条

[1]

DNA sequence evolution in fast evolving mitochondrial DNA nad1 exons in Geraniaceae and Plantaginaceae [J].