A novel adaptor-like protein which is a substrate for the non-receptor tyrosine kinase, BRK

被引：77

作者：

Mitchell, PJ ^{[1
]}

Sara, EA ^{[1
]}

Crompton, MR ^{[1
]}

机构：

[1] Inst Canc Res, Breakthrough Toby Robinson Canc Res Ctr, Sect Cell Biol & Expt Pathol, London SW3 6JB, England

来源：

ONCOGENE | 2000年 / 19卷 / 37期

基金：

英国惠康基金;

关键词：

BKS; BRK; PH domain; tyrosine kinase; SH2; domain;

D O I：

10.1038/sj.onc.1203775

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The brk gene encodes a non-receptor tyrosine kinase that has been found to be overexpressed in approximately two thirds of breast tumours. Using a yeast two-hybrid based screen, we have cloned cDNAs encoding a novel protein, BKS, that is a substrate for the kinase activity of BRK and has the characteristics of an adaptor protein. BKS possesses an N-terminal PH-like domain followed by an SH2-like domain. In co-transfection experiments, high levels of phosphotyrosine were observed on BKS and BRK was found to be associated with BKS, both of which were dependent on the catalytic activity of BRK. The phosphorylation of and association with BKS by BRK was also dependent on the SH2-like domain present within BKS, In addition, BKS recruited an unidentified 100 kDa protein that was also phosphorylated on tyrosine residues in the presence of BRK. We have determined that the BKS protein is expressed in most adult human tissues.

引用

页码：4273 / 4282

页数：10

共 51 条

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