Construction and evaluation of internal control DNA for PCR amplification of Chlamydia trachomatis DNA from urine samples

被引：14

作者：

Betsou, F ^{[1
]}

Beaumont, K ^{[1
]}

Sueur, JM ^{[1
]}

Orfila, J ^{[1
]}

机构：

[1] Biobanque Picardie, F-80000 Amiens, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 03期

关键词：

D O I：

10.1128/JCM.41.3.1274-1276.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An internal control DNA (ICD) with the same primer binding sequences as the target Chlamydia trachomatis DNA was constructed and evaluated in a PCR assay with immunoenzymatic detection. One hundred urine specimens were tested, and 23 were found to contain inhibitors of the PCR, if not subjected to DNA extraction prior to amplification. Coamplification and detection of the ICD appeared to be a useful method for estimating the effects of inhibitors on C. trachomatis DNA amplification.

引用

页码：1274 / 1276

页数：3

共 7 条

[1] CHLAMYDIA-TRACHOMATIS - ITS ROLE IN TUBAL INFERTILITY [J].