Interspecific genetic linkage map, segregation distortion and genetic conversion in coffee (Coffea sp.)

被引:102
作者
Ky, CL
Barre, P
Lorieux, M
Trouslot, P
Akaffou, S
Louarn, J
Charrier, A
Hamon, S
Noirot, M
机构
[1] GeneTrop, Ctr IRD Montepllier, F-34032 Montpellier 1, France
[2] Stn ORSTOM Genet Cafeier, Man, Cote Ivoire
[3] ENSA, UFR Genet & Ameliorat Plantes, F-34060 Montpellier, France
关键词
Coffea sp; AFLPs; RFLPs; linkage map; segregation distortion; genetic conversion;
D O I
10.1007/s001220051529
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
An interspecific partial genetic linkage map of Coffea sp. based on 62 backcross hybrids is presented. F-1 hybrids were generated by a cross between the wild C. pseudozanguebariae and the anciently cultivated C. liberica var. dewevrei (DEW); progeny were then derived from a backcross between F-1 hybrid and DEW. The map construction consisted of a two-step strategy using 5.5 and 3.1 LOD scores revealed by simulation file. The map consisted of 181 loci: 167 amplified fragment length polymorphism (AFLP) and 13 random fragment length polymorphism (RFLP) loci. The markers were as sembled into 14 linkage groups, each with 4-31 markers covering 1,144 cM. Segregation distortion was observed for 30% of all loci, in particular 3:1 and 1:3 ratios equally favouring each of the two parents. The existence of such ratios suggests genetic conversion events. This map also represents an initial step towards the detection of quantitative trait loci.
引用
收藏
页码:669 / 676
页数:8
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