ALS3 encodes a phloem-localized ABC transporter-like protein that is required for aluminum tolerance in Arabidopsis

被引:264
作者
Larsen, PB [1 ]
Geisler, MJB [1 ]
Jones, CA [1 ]
Williams, KM [1 ]
Cancel, JD [1 ]
机构
[1] Univ Calif Riverside, Dept Biochem, Riverside, CA 92521 USA
关键词
aluminum; aluminium; ALS3; ABC transporter; Arabidopsis;
D O I
10.1111/j.1365-313X.2004.02306.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Aluminum (Al) toxicity in acid soils is a worldwide agricultural problem that severely limits crop productivity through inhibition of root growth. Previously, Arabidopsis mutants with increased Al sensitivity were isolated in order to identify genes important for Al tolerance in plants. One mutant, als3, exhibited extreme root growth inhibition in the presence of Al, suggesting that this mutation negatively impacts a gene required for Al tolerance. Map-based cloning of the als3-1 mutation resulted in the isolation of a novel gene that encodes a previously undescribed ABC transporter-like protein, which is highly homologous to a putative bacterial metal resistance protein, ybbM. Northern analysis for ALS3 expression revealed that it is found in all organs examined, which is consistent with the global nature of Al sensitivity displayed by als3, and that expression increases in roots following Al treatment. Based on GUS fusion and in situ hybridization analyses, ALS3 is primarily expressed in leaf hydathodes and the phloem throughout the plant, along with the root cortex following Al treatment. Immunolocalization indicates that ALS3 predominantly accumulates in the plasma membrane of cells that express ALS3. From our results, it appears that ALS3 encodes an ABC transporter-like protein that is required for Al resistance/tolerance and may function to redistribute accumulated Al away from sensitive tissues in order to protect the growing root from the toxic effects of Al.
引用
收藏
页码:353 / 363
页数:11
相关论文
共 33 条
[1]   ASSIGNMENT OF 30 MICROSATELLITE LOCI TO THE LINKAGE MAP OF ARABIDOPSIS [J].
BELL, CJ ;
ECKER, JR .
GENOMICS, 1994, 19 (01) :137-144
[2]   Alterations in the cytoskeleton accompany aluminum-induced growth inhibition and morphological changes in primary roots of maize [J].
Blancaflor, EB ;
Jones, DL ;
Gilroy, S .
PLANT PHYSIOLOGY, 1998, 118 (01) :159-172
[3]  
Boudonck K, 1998, J CELL SCI, V111, P3687
[4]   Aluminum resistance in the Arabidopsis mutant alr-104 is caused by an aluminum-induced increase in rhizosphere pH [J].
Degenhardt, J ;
Larsen, PB ;
Howell, SH ;
Kochian, LV .
PLANT PHYSIOLOGY, 1998, 117 (01) :19-27
[5]   ALUMINUM TOLERANCE IN WHEAT (TRITICUM-AESTIVUM L) .2. ALUMINUM-STIMULATED EXCRETION OF MALIC-ACID FROM ROOT APICES [J].
DELHAIZE, E ;
RYAN, PR ;
RANDALL, PJ .
PLANT PHYSIOLOGY, 1993, 103 (03) :695-702
[6]   ALUMINUM INDUCES RIGOR WITHIN THE ACTIN NETWORK OF SOYBEAN CELLS [J].
GRABSKI, S ;
SCHINDLER, M .
PLANT PHYSIOLOGY, 1995, 108 (03) :897-901
[7]   ALUMINUM INTERACTION WITH PHOSPHOINOSITIDE-ASSOCIATED SIGNAL-TRANSDUCTION [J].
HAUG, A ;
SHI, B ;
VITORELLO, V .
ARCHIVES OF TOXICOLOGY, 1994, 68 (01) :1-7
[8]   Aluminum interactions with voltage-dependent calcium transport in plasma membrane vesicles isolated from roots of aluminum-sensitive and -resistant wheat cultivars [J].
Huang, JWW ;
Pellet, DM ;
Papernik, LA ;
Kochian, LV .
PLANT PHYSIOLOGY, 1996, 110 (02) :561-569
[9]  
HUANG JWW, 1992, PLANTA, V188, P414, DOI 10.1007/BF00192809
[10]  
Jenik PD, 2000, DEVELOPMENT, V127, P1267