Monitoring molecular beacon/DNA interactions using atomic force microscopy

被引：27

作者：

Jin, Y ^{[1
]}

Wang, KM ^{[1
]}

Tan, WH ^{[1
]}

Wu, P ^{[1
]}

Wang, Q ^{[1
]}

Huang, HM ^{[1
]}

Huang, SS ^{[1
]}

Tang, ZW ^{[1
]}

Guo, QP ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, Ctr Biomed Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 19期

关键词：

D O I：

10.1021/ac049933u

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The molecular beacon (MB) is anew fluorescence probe containing a single-stranded oligonucleotide with a probe sequence embedded in complementary sequences that form a hairpin stem. Due to the inherent fluorescent signal transduction mechanism, an MB functions as a sensitive probe with a high signal-to-background ratio for real-time monitoring and provides a variety of exciting opportunities in DNA, RNA, and protein studies. To better understand the properties of MBs, the specific interactions between MB and target DNA (complementary and one-base mismatch) have been directly investigated by atomic force microscopy. The interaction force between a linear DNA probe and the target DNA was also detected and compared to that between MB and target DNA. The results demonstrate the high specificity of the MB/target DNA compared to the linear DNA/target DNA interaction.

引用

页码：5721 / 5725

页数：5

共 33 条

[1] DIRECT MEASUREMENT OF HYDROGEN-BONDING IN DNA NUCLEOTIDE BASES BY ATOMIC-FORCE MICROSCOPY [J].