Molecular cloning of the dog beta 1 and beta 2 adrenergic receptors

被引：6

作者：

Huang, RRC ^{[1
]}

Rapoport, D ^{[1
]}

Schaeffer, MT ^{[1
]}

Cascieri, MA ^{[1
]}

Fong, TM ^{[1
]}

机构：

[1] MERCK & CO INC,MERCK SHARP & DOHME RES LABS,RAHWAY,NJ 07065

来源：

JOURNAL OF RECEPTOR AND SIGNAL TRANSDUCTION RESEARCH | 1997年 / 17卷 / 04期

关键词：

D O I：

10.3109/10799899709039152

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the isolation of the genes encoding the beta 1 and beta 2 adrenergic receptors from dog genomic DNA. Sequence analysis of both genes revealed intronless open reading frames of 473 and 415 amino acid residues, receptively. Heterologous expression of both receptors in CHO cells indicated that both receptors are functionally similar to the human homologs. Comparing the dog beta 1 and beta 2 adrenergic receptors, the beta 1 receptor appears to bind to G proteins more tightly than the beta 2 receptor. Heterologously expressed receptors provide a convenient system for evaluating novel receptor agonists and antagonists.

引用

页码：599 / 607

页数：9

共 12 条

[1] CLONING, SEQUENCING, AND EXPRESSION OF THE RHESUS-MONKEY BETA(2) ADRENERGIC-RECEPTOR [J].

AMEND, AM ;

GUAN, XM .

DNA AND CELL BIOLOGY, 1995, 14 (09) :753-757

[2] ATYPICAL BETA-ADRENOCEPTOR ON BROWN ADIPOCYTES AS TARGET FOR ANTI-OBESITY DRUGS [J].

ARCH, JRS ;

AINSWORTH, AT ;

CAWTHORNE, MA ;

PIERCY, V ;

SENNITT, MV ;

THODY, VE ;

WILSON, C ;

WILSON, S .

NATURE, 1984, 309 (5964) :163-165

[3]

CHEUNG AH, 1992, MOL PHARMACOL, V41, P1061

[4] CALCULATING RECEPTOR NUMBER FROM BINDING EXPERIMENTS USING SAME COMPOUND AS RADIOLIGAND AND COMPETITOR [J].

DEBLASI, A ;

OREILLY, K ;

MOTULSKY, HJ .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1989, 10 (06) :227-229

[5] CLONING OF THE GENE AND CDNA FOR MAMMALIAN BETA-ADRENERGIC-RECEPTOR AND HOMOLOGY WITH RHODOPSIN [J].

DIXON, RAF ;

KOBILKA, BK ;

STRADER, DJ ;

BENOVIC, JL ;

DOHLMAN, HG ;

FRIELLE, T ;

BOLANOWSKI, MA ;

BENNETT, CD ;

RANDS, E ;

DIEHL, RE ;

MUMFORD, RA ;

SLATER, EE ;

SIGAL, IS ;

CARON, MG ;

LEFKOWITZ, RJ ;

STRADER, CD .

NATURE, 1986, 321 (6065) :75-79

[6] MOLECULAR CHARACTERIZATION OF THE HUMAN BETA-3-ADRENERGIC RECEPTOR [J].

EMORINE, LJ ;

MARULLO, S ;

BRIENDSUTREN, MM ;

PATEY, G ;

TATE, K ;

DELAVIERKLUTCHKO, C ;

STROSBERG, AD .

SCIENCE, 1989, 245 (4922) :1118-1121

[7] CLONING OF THE CDNA FOR THE HUMAN BETA-1-ADRENERGIC RECEPTOR [J].

FRIELLE, T ;

COLLINS, S ;

DANIEL, KW ;

CARON, MG ;

LEFKOWITZ, RJ ;

KOBILKA, BK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (22) :7920-7924

[8]

FRIELLE T, 1988, TRENDS NEUROSCI, V7, P321

[9]

HOWE R, 1992, J MED CHEM, V35, P1759, DOI 10.1021/jm00088a010

[10] THE RHESUS MACAQUE BETA-1-ADRENERGIC RECEPTOR GENE - STRUCTURE OF THE GENE AND COMPARISON OF THE FLANKING SEQUENCES WITH THE RAT BETA-1-ADRENERGIC RECEPTOR GENE [J].

SEARLES, RP ;

NIPPER, VJ ;

MACHIDA, CA .

DNA SEQUENCE, 1994, 4 (04) :231-241

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