Identification of the Y985 and Y1077 motifs as SOCS3 recruitment sites in the murine leptin receptor

被引：118

作者：

Eyckerman, S ^{[1
]}

Broekaert, D ^{[1
]}

Verhee, A ^{[1
]}

Vandekerckhove, J ^{[1
]}

Tavernier, J ^{[1
]}

机构：

[1] State Univ Ghent VIB, Fac Med & Hlth Sci, Dept Med Prot Res, B-9000 Ghent, Belgium

来源：

FEBS LETTERS | 2000年 / 486卷 / 01期

关键词：

leptin receptor; signal transduction; suppressor of cytokine signalling;

D O I：

10.1016/S0014-5793(00)02205-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The leptin system provides a link between adipose mass and the central nervous system. The appetite suppressing effects of leptin are impaired in most obese patients and some mutant mice strains. Herein,ve describe how suppressor of cytokine signalling 3 (SOCS3), a potential mediator of this leptin resistance is recruited into the activated murine leptin receptor complex. Using a functional assay based on inhibition of leptin mediated reporter induction, and using phosphopeptide affinity chromatography we show binding of SOCS3 to the highly conserved phosphorylated Tyr-985 and Tyr-1077 motifs within the mouse leptin receptor. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：33 / 37

页数：5

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