Deep sequencing analysis of viruses infecting grapevines: Virome of a vineyard

被引：188

作者：

Coetzee, Beatrix ^{[1
]}

Freeborough, Michael-John ^{[1
]}

Maree, Hans J. ^{[1
]}

Celton, Jean-Marc ^{[2
]}

Rees, D. Jasper G. ^{[2
]}

Burger, Johan T. ^{[1
]}

机构：

[1] Univ Stellenbosch, Dept Genet, ZA-7602 Stellenbosch, South Africa

[2] Univ Western Cape, Dept Biotechnol, ZA-7535 Bellville, South Africa

来源：

VIROLOGY | 2010年 / 400卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Metagenomic sequencing; Deep sequencing analysis; Grapevine viruses; DOUBLE-STRANDED RNAS; SHIRAZ DISEASE; VARIANTS; REVEALS; GENERATION; GVA;

D O I：

10.1016/j.virol.2010.01.023

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Double stranded RNA, isolated from 44 pooled randomly selected vines from a diseased South African vineyard, has been used in a deep sequencing analysis to build a census of the viral population. The dsRNA was sequenced in an unbiased manner using the sequencing-by-synthesis technology offered by the Illumina Genome Analyzer II and yielded 837 megabases of metagenomic sequence data. Four known viral pathogens were identified. It was found that Grapevine leafroll-associated virus 3 (GLRaV-3) is the most prevalent species, constituting 59% of the total reads, followed by Grapevine rupestris stem pitting-associated virus and Grapevine virus A. Grapevine virus E, a virus not previously reported in South African vineyards, was identified in the census. Viruses not previously identified in grapevine were also detected. The second most prevalent virus detected was a member of the Chrysoviridae family similar to Penicillium chrysogenum virus. Sequences aligning to two other mycoviruses were also detected. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：157 / 163

页数：7

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