Three insertion sequences from the cyanobacterium Synechocystis PCC6803 support the occurrence of horizontal DNA transfer among bacteria

被引:29
作者
CassierChauvat, C
Poncelet, M
Chauvat, F
机构
[1] CEA SACLAY, SERV BIOCHIM & GENET MOL, F-91191 GIF SUR YVETTE, FRANCE
[2] CEA SACLAY, URA 2096 CNRS, F-91191 GIF SUR YVETTE, FRANCE
关键词
mobile elements; Tc1-mariner; IS4; family; target duplication; copy number; horizontal transfer;
D O I
10.1016/S0378-1119(97)00165-0
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Three insertion sequences were characterized from the widely-used cyanobacterium Synechocystis PCC6803. They all harbored a putative transposase sequence flanked by two imperfect inverted repeats, seemed to have duplicated their target insertion site and occurred as multiple copies in the host genome. They exhibited no obvious homology with any other cyanobacterial ISs and were termed IS5S (871 bp), IS4S (1299 bp) and ISS1987 (949 bp) because they were, respectively, homologous to IS5- and IS4-bacterial elements, and to several members of the IS63O-Tcl-mariner superfamily of IS elements occurring in a wide range of hosts. This suggests that these IS-elements were spread through horizontal transfer between evolutionary distant organisms. Three IS5S-copies were isolated as a rescue insertion into a replicating plasmid (IS5Sa), or subsequently cloned from a Synechocystis DNA-library probed with IS5Sa (IS5Sb and IS5Sc), and appeared to be almost identical. In the vicinity of IS5Sb, we found the ISS1987 element inserted into the ISIS element. This indicates that the ISS1987 element has been, and could still be, mobile since its transposase sequence is not interrupted with stop codons or translational frameshifts, unlike that which is found in most members of the IS63O-Tcl-mariner superfamily of transposable elements. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:257 / 266
页数:10
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