Amyloid β concentrations and stable isotope labeling kinetics of human plasma specific to central nervous system amyloidosis

被引：435

作者：

Ovod, Vitaliy ^{[1
]}

Ramsey, Kara N. ^{[1
]}

Mawuenyega, Kwasi G. ^{[1
]}

Bollinger, Jim G. ^{[1
]}

Hicks, Terry ^{[1
]}

Schneider, Theresa ^{[1
]}

Sullivan, Melissa ^{[1
]}

Paumier, Katrina ^{[1
]}

Holtzman, David M. ^{[1
,2
,3
]}

Morris, John C. ^{[1
,3
]}

Benzinger, Tammie ^{[3
,4
]}

Fagan, Anne M. ^{[1
,2
,3
]}

Patterson, Bruce W. ^{[5
]}

Bateman, Randall J. ^{[1
,2
,3
]}

机构：

[1] Washington Univ, Sch Med, Dept Neurol, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Dept Neurol, Hope Ctr Neurol Disorders, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Dept Neurol, Knight Alzheimers Dis Res Ctr, St Louis, MO 63110 USA

[4] Washington Univ, Sch Med, Dept Radiol, St Louis, MO USA

[5] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA

来源：

ALZHEIMERS & DEMENTIA | 2017年 / 13卷 / 08期

关键词：

Amyloid beta; A beta 42; Turnover; Kinetics; Human; Plasma; CEREBROSPINAL-FLUID TAU; ALZHEIMERS-DISEASE; AGE; BIOMARKERS; DIAGNOSIS; A-BETA-42;

D O I：

10.1016/j.jalz.2017.06.2266

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Introduction: Cerebrospinal fluid analysis and other measurements of amyloidosis, such as amyloid-binding positron emission tomography studies, are limited by cost and availability. There is a need for a more practical amyloid beta (A beta) biomarker for central nervous system amyloid deposition. Methods: We adapted our previously reported stable isotope labeling kinetics protocol to analyze the turnover kinetics and concentrations of A beta 38, A beta 40, and A beta 42 in human plasma. Results: A beta isoforms have a half-life of approximately 3 hours in plasma. A beta 38 demonstrated faster turnover kinetics compared with A beta 40 and A beta 42. Faster fractional turnover of A beta 42 relative to A beta 40 and lower A beta 42 and A beta 42/A beta 40 concentrations in amyloid-positive participants were observed. Discussion: Blood plasma A beta 42 shows similar amyloid-associated alterations as we have previously reported in cerebrospinal fluid, suggesting a blood-brain transportation mechanism of A beta. The stability and sensitivity of plasma A beta measurements suggest this may be a useful screening test for central nervous system amyloidosis. (C) 2017 the Alzheimer's Association. Published by Elsevier Inc. All rights reserved.

引用

页码：841 / 849

页数：9

共 17 条

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