Recovery of homogeneous and functional β2-adrenergic receptors from extracellular baculovirus particles

被引：62

作者：

Loisel, TP

Ansanay, H

St-Onge, S

Gay, B

Boulanger, P

Strosberg, AD

Marullo, S

Bouvier, M ^{[1
]}

机构：

[1] Univ Montreal, Dept Biochim, Montreal, PQ H3C 3J7, Canada

[2] Univ Montreal, Grp Rech Syst Nerveux Autonome, Montreal, PQ H3C 3J7, Canada

[3] Fac Med Montpellier, Inst Biol, Lab Virol & Pathogeneses Mol, CNRS,URA 1487, F-34060 Montpellier, France

[4] CNRS, Inst Cochin Genet Mol, UPR 415, Lab Immunopharmacol Mol, F-75014 Paris, France

[5] Univ Paris 07, F-75014 Paris, France

来源：

NATURE BIOTECHNOLOGY | 1997年 / 15卷 / 12期

关键词：

protein expression; Sf9; cells; recombinant baculovirus;

D O I：

10.1038/nbt1197-1300

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Expression in baculovirus-infected insect cells allows sufficient production of G-protein coupled receptor for structural studies. An important drawback of this expression system comes from the presence of unprocessed and biologically inactive receptors that have to be eliminated during receptor purification steps. We show that viral particles released from Sf9 cells infected with a recombinant baculovirus coding for the human beta(2)-adrenergic receptor (beta(2)AR) cDNA contain glycosylated and biologically active beta(2)AR. In addition, post-translational modifications known to modulate receptor activity were found to occur in these particles.

引用

页码：1300 / 1304

页数：5

共 39 条

[1] SEQUENCE OF THE GENE ENCODING THE HUMAN M1 MUSCARINIC ACETYLCHOLINE-RECEPTOR [J].