Stereoselective reduction of ethyl 4-chloro acetoacetate with recombinant Pichia pastoris

被引：33

作者：

Engelking, H

Pfaller, R

Wich, G

Weuster-Botz, D

机构：

[1] Tech Univ Munich, Lehrstuhl Bioverfahrenstech, D-85748 Garching, Germany

[2] Consortium Elektrochem Ind GmbH, D-81379 Munich, Germany

来源：

TETRAHEDRON-ASYMMETRY | 2004年 / 15卷 / 22期

关键词：

D O I：

10.1016/j.tetasy.2004.09.021

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

A recombinant strain of Pichia pastoris coexpressing the carbonyl reductase of Candida magnoliae and the glucose dehydrogenase of Bacillus subtilis was used for the stereoselective reduction of ethyl 4-chloro acetoacetate 1 to (S)-4-chloro-3-hydroxybutanoate 2. The bioreduction was performed in a two-phase reaction system with n-butyl acetate as organic solvent. Complete conversion of 350 mM 1 with a final yield of 91% and an enantiomeric excess of 95% was achieved by continuous feeding of cell suspension and ketoester 1. (C) 2004 Elsevier Ltd. All rights reserved.

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页码：3591 / 3593

页数：3

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