Protective effect of lycopene on lipid peroxidation and oxidative DNA damage in cell culture

被引：116

作者：

Matos, HR ^{[1
]}

Di Mascio, P ^{[1
]}

Medeiros, MHG ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05513970 Sao Paulo, SP, Brazil

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2000年 / 383卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

lycopene; DNA damage; 8-oxo-7,8-dihydro-2 '-deoxyguanosine; lipoperoxidation; Fe-NTA;

D O I：

10.1006/abbi.2000.2035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A high incidence of cancer has been correlated with chronic iron overload, and carotenoids are of interest as possible anticarcinogens. We have investigated the effect of lycopene on lipid peroxidation and on the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in CV1-P monkey cells exposed to ferric nitrilotriacetate (Fe-NTA) plus ascorbate. Cells supplemented with lycopene (20 pmol/10(6) cells) showed a reduction of 86% in Fe-NTA/ascorbate-induced lipid peroxidation (TBARS). Levels of 8-oxodGuo rose from 1.59 +/- 0.09 residues/10(6) dGuo in the control cells to 14.02 +/- 0.41 residues/10(6) dGuo after incubation with (1:4 mM) Fe-NTA/ascorbate (40 muM). Lycopene supplementation decreased in 77% the 8-oxodGuo levels in Fe-NTA/ascorbate-treated cells. These results indicate that lycopene can protect mammalian cells against membrane and DNA damage and possibly play a protective role against tumor promotion associated with oxidative damage. (C) 2000 Academic Press.

引用

页码：56 / 59

页数：4

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