Rapid Detection of Influenza A Pandemic (H1N1) 2009 Virus Neuraminidase Resistance Mutation H275Y by Real-Time Reverse Transcriptase PCR

被引:34
作者
Hindiyeh, Musa [1 ]
Ram, Daniela [1 ]
Mandelboim, Michal [1 ,2 ]
Meningher, Tal [2 ]
Hirsh, Shira [2 ]
Robinov, Jana [1 ]
Levy, Virginia [1 ]
Orzitzer, Sara [1 ]
Azar, Roberto [1 ]
Grossman, Zehava [1 ,3 ]
Mendelson, Ella [1 ,3 ]
机构
[1] Chaim Sheba Med Ctr, Cent Virol Lab, Minist Hlth, Publ Hlth Serv, IL-52621 Tel Hashomer, Israel
[2] Bar Ilan Univ, Fac Life Sci, Ramat Gan, Israel
[3] Tel Aviv Univ, Sackler Fac Med, Sch Publ Hlth, Dept Epidemiol & Prevent Med, IL-69978 Tel Aviv, Israel
关键词
OSELTAMIVIR-RESISTANCE; INHIBITORS;
D O I
10.1128/JCM.02540-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.
引用
收藏
页码:1884 / 1887
页数:4
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