Rapid Detection of Influenza A Pandemic (H1N1) 2009 Virus Neuraminidase Resistance Mutation H275Y by Real-Time Reverse Transcriptase PCR

被引：34

作者：

Hindiyeh, Musa ^{[1
]}

Ram, Daniela ^{[1
]}

Mandelboim, Michal ^{[1
,2
]}

Meningher, Tal ^{[2
]}

Hirsh, Shira ^{[2
]}

Robinov, Jana ^{[1
]}

Levy, Virginia ^{[1
]}

Orzitzer, Sara ^{[1
]}

Azar, Roberto ^{[1
]}

Grossman, Zehava ^{[1
,3
]}

Mendelson, Ella ^{[1
,3
]}

机构：

[1] Chaim Sheba Med Ctr, Cent Virol Lab, Minist Hlth, Publ Hlth Serv, IL-52621 Tel Hashomer, Israel

[2] Bar Ilan Univ, Fac Life Sci, Ramat Gan, Israel

[3] Tel Aviv Univ, Sackler Fac Med, Sch Publ Hlth, Dept Epidemiol & Prevent Med, IL-69978 Tel Aviv, Israel

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 05期

关键词：

OSELTAMIVIR-RESISTANCE; INHIBITORS;

D O I：

10.1128/JCM.02540-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.

引用

页码：1884 / 1887

页数：4

共 18 条

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