Crosslinked collagen/chitosan matrix for artificial livers

被引：361

作者：

Wang, XH

Li, DP

Wang, WJ

Feng, QL ^{[1
]}

Cui, FZ

Xu, YX

Song, XH

van der Werf, M

机构：

[1] Tsing Hua Univ, Dept Mat Sci & Engn, Beijing 100084, Peoples R China

[2] Chinese Peoples Liberat Army Gen Hosp, Inst Gen Surg, Beijing 100853, Peoples R China

[3] Univ Twente, Dept Mech Engn, NL-7500 AE Enschede, Netherlands

来源：

BIOMATERIALS | 2003年 / 24卷 / 19期

关键词：

collagen/chitosan matrix (CCM); 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC); hepatocyte; liver;

D O I：

10.1016/S0142-9612(03)00170-4

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 [生物医学工程];

摘要：

Matrices composed of collagen and chitosan may create an appropriate environment for the regeneration of livers. In this study, we have prepared, characterized and evaluated a new collagen/chitosan matrix (CCM). The CCM was made by using crosslinking agent 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) in N-hydroxysuccinimide (NHS) and a 2-morpholinoethane sulfonic acid (MES) buffer system. The chemical characteristics were evaluated by Fourier-transformed infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The mechanical strength was measured by tensile tests. The platelet deposition and hepatocyte culture experiments show that CCM has excellent blood and cell compatibility. The results suggest that the CCM is a promising candidate matrix for implantable bioartificial livers. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：3213 / 3220

页数：8

共 33 条

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Chitosan microcapsules as controlled release systems for insulin [J].