Novel mouse type D endogenous proviruses and ETn elements share long terminal repeat and internal sequences

被引:46
作者
Mager, DL
Freeman, JD
机构
[1] British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada
[2] Univ British Columbia, Dept Med Genet, Vancouver, BC V5Z 1M9, Canada
关键词
D O I
10.1128/JVI.74.16.7221-7229.2000
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The repetitive ETn (early transposon) family of sequences represents an active " mobile mutagen" in the mouse genome. The presence of long terminal repeats (LTRs) and other diagnostic features indicate that ETns are retrotransposons but they contain no long open reading frames or documented similarity to the genes of known retroviruses or other retroelements. Thus, the mechanisms responsible for the mobility of this family have been unknown. in this study, we used computer searches to detect a small region of previously unrecognized type D retroviral pol homology within ETn elements. This small region was used to isolate two mouse endogenous proviral elements with gag, pro, and pol genes similar to simian type D viruses. This new family of mouse endogenous proviruses, termed MusD, is present in several hundred copies in the genome. Interestingly, the MusD LTRs, 3' internal region, and the 5' region expected to contain the packaging signal are very closely related to members of the ETn subfamily that have recently transposed. Analysis of different mouse strains indicates that MusD elements predate the existence of the mobile subfamily of ETns, These findings indicate that the ETn family was likely created via recombination events resulting in a near complete substitution of MusD coding sequences with unrelated DNA. Furthermore, these results suggest that ETn transcripts retrotranspose using proteins provided by MusD proviruses.
引用
收藏
页码:7221 / 7229
页数:9
相关论文
共 30 条
[1]   ABERRANT TRANSCRIPTION CAUSED BY THE INSERTION OF AN EARLY TRANSPOSABLE ELEMENT IN AN INTRON OF THE FAS ANTIGEN GENE OF LPR MICE [J].
ADACHI, M ;
WATANABEFUKUNAGA, R ;
NAGATA, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (05) :1756-1760
[2]   Genealogies of mouse inbred strains [J].
Beck, JA ;
Lloyd, S ;
Hafezparast, M ;
Lennon-Pierce, M ;
Eppig, JT ;
Festing, MFW ;
Fisher, EMC .
NATURE GENETICS, 2000, 24 (01) :23-+
[3]   SPATIAL-DISTRIBUTION OF TRANSCRIPTS OF THE LONG REPEATED ETN SEQUENCE DURING EARLY MOUSE EMBRYOGENESIS [J].
BRULET, P ;
CONDAMINE, H ;
JACOB, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (07) :2054-2058
[4]   EARLY DIFFERENTIAL TISSUE EXPRESSION OF TRANSPOSON-LIKE REPETITIVE DNA-SEQUENCES OF THE MOUSE [J].
BRULET, P ;
KAGHAD, M ;
XU, YS ;
CROISSANT, O ;
JACOB, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (18) :5641-5645
[6]   ORIGINS AND EVOLUTIONARY RELATIONSHIPS OF RETROVIRUSES [J].
DOOLITTLE, RF ;
FENG, DF ;
JOHNSON, MS ;
MCCLURE, MA .
QUARTERLY REVIEW OF BIOLOGY, 1989, 64 (01) :1-30
[7]   IDENTIFICATION OF CONSERVED AMINO-ACID-RESIDUES CRITICAL FOR HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INTEGRASE FUNCTION-INVITRO [J].
ENGELMAN, A ;
CRAIGIE, R .
JOURNAL OF VIROLOGY, 1992, 66 (11) :6361-6369
[8]   SECONDARY STRUCTURE MODEL OF THE MASON-PFIZER MONKEY VIRUS 5' LEADER SEQUENCE - IDENTIFICATION OF A STRUCTURAL MOTIF COMMON TO A VARIETY OF RETROVIRUSES [J].
HARRISON, GP ;
HUNTER, E ;
LEVER, AML .
JOURNAL OF VIROLOGY, 1995, 69 (04) :2175-2186
[9]   CLONING OF THE T-GENE REQUIRED IN MESODERM FORMATION IN THE MOUSE [J].
HERRMANN, BG ;
LABEIT, S ;
POUSTKA, A ;
KING, TR ;
LEHRACH, H .
NATURE, 1990, 343 (6259) :617-622
[10]   Spontaneous mutations in SELH/Bc mice due to insertions of early transposons:: Molecular characterization of null alleles at the nude and albino loci [J].
Hofmann, M ;
Harris, M ;
Juriloff, D ;
Boehm, T .
GENOMICS, 1998, 52 (01) :107-109