Circadian profile and photic regulation of clock genes in the suprachiasmatic nucleus of a diurnal mammal Arvicanthis ansorgei

被引:104
作者
Caldelas, I
Poirel, VJ
Sicard, B
Pévet, P
Challet, E [1 ]
机构
[1] Univ Strasbourg, CNRS UMR7518, Lab Neurobiol Rhythms, F-67000 Strasbourg, France
[2] Inst Rech Dev, Lab Mammal, Bamako, Mali
[3] Res Ctr Populat Biol & Management, CBGP, F-34988 Montferrier Sur Lez, France
关键词
light; period genes; cryptochrome genes; circadian rhythms; diurnality;
D O I
10.1016/S0306-4522(02)00654-1
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The molecular mechanisms of the mammalian circadian clock located in the suprachiasmatic nucleus have been essentially studied in nocturnal species. Currently, it is not clear if the clockwork and the synchronizing mechanisms are similar between diurnal and nocturnal species. Here we investigated in a day-active rodent Arvicanthis ansorgei, some of the molecular mechanisms that participate in the generation of circadian rhythmicity and processing of photic signals. In situ hybridization was used to characterize circadian profiles of expression of Per1, Per2, Cry2 and Bmal1 in the suprachiasmatic nucleus of A. ansorgei housed in constant dim red light. All the clock genes studied showed a circadian expression. Pert and Pert mRNA increased during the subjective day and decreased during the subjective night. Also, Bmal1 exhibited a circadian expression, but in anti-phase to that of Pert. The expression of Cry2 displayed a circadian pattern, increasing during the late subjective day and decreasing during the late subjective night. We also obtained the phase responses to light for wheel-running rhythm and clock gene expression. At a behavioral level, light was able to induce phase shifts only during the subjective night, like in other diurnal and nocturnal species. At a molecular level, light pulse exposure during the night led to an up-regulation of Per1 and Per2 concomitant with a down-regulation of Cry2 in the suprachiasmatic nucleus of A. ansorgei. In contrast, Bmal1 expression was not affected by light pulses at the circadian times investigated. This study demonstrates that light exposure during the subjective night has opposite effects on the expression of the clock genes Per1 and Per2 compared with that of Cry2. These differential effects can participate in photic resetting of the circadian clock. Our data also indicate that the molecular mechanisms underlying circadian rhythmicity and photic synchronization share clear similarities between diurnal and nocturnal mammals. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:583 / 591
页数:9
相关论文
共 47 条
[1]   Circadian rhythm and light responsiveness of BMAL1 expression, a partner of mammalian clock gene Clock, in the suprachiasmatic nucleus of rats [J].
Abe, H ;
Honma, S ;
Namihira, M ;
Tanahashi, Y ;
Ikeda, M ;
Honma, K .
NEUROSCIENCE LETTERS, 1998, 258 (02) :93-96
[2]   Suprachiasmatic nucleus in the mouse: retinal innervation, intrinsic organization and efferent projections [J].
Abrahamson, EE ;
Moore, RY .
BRAIN RESEARCH, 2001, 916 (1-2) :172-191
[3]  
Akiyama M, 1999, J NEUROSCI, V19, P1115
[4]   mPer1 and mPer2 are essential for normal resetting of the circadian clock [J].
Albrecht, U ;
Zheng, BH ;
Larkin, D ;
Sun, ZS ;
Lee, CC .
JOURNAL OF BIOLOGICAL RHYTHMS, 2001, 16 (02) :100-104
[5]   A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light [J].
Albrecht, U ;
Sun, ZS ;
Eichele, G ;
Lee, CC .
CELL, 1997, 91 (07) :1055-1064
[6]   Differential functions of mPer1, mPer2, and mPer3 in the SCN circadian clock [J].
Bae, K ;
Jin, XW ;
Maywood, ES ;
Hastings, MH ;
Reppert, SM ;
Weaver, DR .
NEURON, 2001, 30 (02) :525-536
[7]   Nocturnal and diurnal rhythms in the unstriped Nile rat, Arvicanthis niloticus [J].
Blanchong, JA ;
McElhinny, TL ;
Mahoney, MM ;
Smale, L .
JOURNAL OF BIOLOGICAL RHYTHMS, 1999, 14 (05) :364-377
[8]   Mop3 is an essential component of the master circadian pacemaker in mammals [J].
Bunger, MK ;
Wilsbacher, LD ;
Moran, SM ;
Clendenin, C ;
Radcliffe, LA ;
Hogenesch, JB ;
Simon, MC ;
Takahashi, JS ;
Bradfield, CA .
CELL, 2000, 103 (07) :1009-1017
[9]   Circadian organization in a diurnal rodent, Arvicanthis ansorgei Thomas 1910:: Chronotypes, responses to constant lighting conditions, and photoperiodic changes [J].
Challet, E ;
Pitrosky, B ;
Sicard, B ;
Malan, A ;
Pévet, P .
JOURNAL OF BIOLOGICAL RHYTHMS, 2002, 17 (01) :52-64
[10]   FUNCTIONAL-ANALYSIS OF CIRCADIAN PACEMAKERS IN NOCTURNAL RODENTS .2. VARIABILITY OF PHASE RESPONSE CURVES [J].
DAAN, S ;
PITTENDRIGH, CS .
JOURNAL OF COMPARATIVE PHYSIOLOGY, 1976, 106 (03) :253-266