Overexpression of human erythropoietin (EPO) affects plant morphologies:: retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis

被引:54
作者
Cheon, BY
Kim, HJ
Oh, KH
Bahn, SC
Ahn, JH
Choi, JW
Ok, SH
Bae, JM
Shin, JS [1 ]
机构
[1] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea
[2] Taegu Univ, Coll Nat Resources, Div Life Sci, Kyungsan 712714, South Korea
关键词
Arabidopsis thaliana; dwarfism; erythropoietin (EPO); male sterility; Nicotiana tabacum; overexpression; phenotypic change;
D O I
10.1007/s11248-004-2737-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Erythropoietin (EPO) is a glycoprotein used for curing human anemia by regulating the differentiation of erythroid progenitors and the production of red blood cells. To examine the expression of recombinant EPO in plants, pPEV-EP21, in which human epo cDNA under the control of the CaMV 35S promoter, was introduced into tobacco and Arabidopsisvia Agrobacterium tumefaciens-mediated transformation. The RNA expression level of epo in the transgenic lines was initially estimated by Northern blot analysis. Two transgenic lines, which exhibited a high expression level of epo mRNA determined by Northern analysis, were chosen for Western blot analysis to examine the production of EPO proteins. Those two lines, EP21-12 and EP21-14, revealed detectable bands on the immunoblot. Interestingly, constitutive expression of the human epo gene affected the morphologies in transgenic plants such that vegetative growth of transgenic tobacco was retarded, and male sterility was induced in transgenic tobacco and Arabidopsis.
引用
收藏
页码:541 / 549
页数:9
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