Development of a hydroxyl radical ratiometric nanoprobe

被引：39

作者：

King, M ^{[1
]}

Kopelman, R ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2003年 / 90卷 / 1-3期

关键词：

nanoprobe; PEBBLE; hydroxyl radical;

D O I：

10.1016/S0925-4005(03)00100-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A hydroxyl radical nanoprobe has been developed by covalently attaching coumarin-3-carboxylic acid (CCA) to amine-functionalized polyacrylamide (AFPA) nanoparticles (40-100 nm in size). CCA is a non-fluorescent aromatic compound that reacts with hydroxyl radical ((OH)-O-.) to produce a fluorescent product, 7-hydroxy-CCA (7-OH-CCA). Texas Red-Dextran (a reference dye) is encapsulated within the nanoprobe matrix to allow for ratiometric measurements to be made that correct for variations in source intensity, sample and instrument geometry and dilution. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：76 / 81

页数：6

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